|
|||||
|
|
| 兔脂肪间质干细胞的分离培养与鉴定 | |
| 引用本文： | 马勇江,杨学义,窦忠英,李玉谷,张媛.兔脂肪间质干细胞的分离培养与鉴定[J].中国兽医科学,2006,36(9):733-737. |
| 作者姓名： | 马勇江 杨学义 窦忠英 李玉谷 张媛 |
| 作者单位： | 1. 华南农业大学,兽医学院,广东,广州,510642;西北农林科技大学,陕西省干细胞工程技术研究中心,陕西,杨凌,712100 2. 西北农林科技大学,陕西省干细胞工程技术研究中心,陕西,杨凌,712100 3. 华南农业大学,兽医学院,广东,广州,510642 |
| 基金项目： | 国家高技术研究发展计划(863计划);教育部重大科技攻关项目 |
| 摘 要： | 取兔腹股沟皮下脂肪组织,用Ⅰ型胶原酶消化,分离培养脂肪间质干细胞(MSCs),并用免疫组化和体外诱导分化方法对其表面分子标志和多向分化潜能进行鉴定。结果显示,兔脂肪组织中能够分离培养出脂肪MSCs,该类细胞表达CD29、CD44和CD105,不表达CD34、CD45及HLA-DR表面分子标志,并具有可分化为脂肪细胞、神经细胞和成骨细胞的多向分化潜能,证实兔脂肪组织中存在具有多向分化潜能的MSCs。 |
| 关 键 词： | 脂肪组织 间质干细胞 兔 |
| 文章编号： | 1673-4696(2006)09-0733-05 |
| 修稿时间： | 2006年6月12日 |
Cultivation and identification of adipose tissue-derived mesenchymal stem cells from rabbits |
|
| MA Yong-jiang,YANG Xue-yi,DOU Zhong-ying,LI Yu-gu,ZHANG Yuan.Cultivation and identification of adipose tissue-derived mesenchymal stem cells from rabbits[J].Veterinary Science in China,2006,36(9):733-737. | |
| Authors: | MA Yong-jiang YANG Xue-yi DOU Zhong-ying LI Yu-gu ZHANG Yuan |
| Abstract: | Mesenchymal stem cells isolated from rabbit inguinal fat pads were digested with collagenase type I,and their molecular characteristics and multi-lineage potential were identified by immunohistochemical methods and induction in vitro.In results,rabbit adipose tissue derived mesenchymal stem cells could express CD29,CD44 and CD105,whereas they did not express CD34,CD45 or HLA-DR.They had the(ability) to differentiate into adipocytes,neurocytes and osteoblasts.The results provided significant evidence that there are multipotential mesenchymal stem cells in rabbit adipose tissue. |
| Keywords: | adipose tissue mesenchymal stem cells rabbit |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
