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| 益智仁化学成分的研究 | |
| 引用本文： | 徐俊驹,谭宁华,曾广智,韩洪金,黄火强,嵇长久.益智仁化学成分的研究[J].中国中药杂志,2009,34(8):990. |
| 作者姓名： | 徐俊驹 谭宁华 曾广智 韩洪金 黄火强 嵇长久 |
| 作者单位： | 1. 中国科学院,昆明植物研究所,植物化学与西部植物资源持续利用国家重点实验室,云南,昆明,650204;中国科学院,研究生院,北京,100049 2. 中国科学院,昆明植物研究所,植物化学与西部植物资源持续利用国家重点实验室,云南,昆明,650204 |
| 基金项目： | 国家杰出青年科学基金项目(30725048)；国家重点基础研究发展计划(973)项目 |
| 摘 要： | 目的:研究益智仁Alpinia oxyphylla的化学成分.方法:采用硅胶和HPLC等色谱手段进行化学成分的分离纯化,根据波谱数据结合理化性质鉴定化合物结构;用SRB法对化合物进行肿瘤细胞毒活性试验.结果:从益智仁的70%丙酮-水提取物中分得8个化合物,鉴定其结构分别为(9E)-humulene-2,3;6,7-diepoxide(1),3(12),7(13),9(E).humulatriene-2,6-diol(2),(-)-oplopanone(3),yakuchinone A(4),yakuchinone B(S),杨芽黄素(6),异香草醛(7),(2E,4E)-6-羟基-2,6-二甲基-2,4-庚二烯醛(8);用肿瘤细胞株A549,HT-29和SGC-7901测定了化合物1,3～5的细胞毒活性.结论:化合物1～3,7,8为首次从该属植物中分离得到;化合物1,3～5 10 mg·L~(-1)对肿瘤细胞株A549,HT-29和SGC-7901未显示出细胞毒活性. |
| 关 键 词： | 姜科 益智仁 化学成分 |
Studies on chemical constituents in fruit of Alpinia oxyphylla |
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| XU Dun-Ju,Tan-Ning-Hu,Ceng-An-Zhi,Han-Hong-Jin,Huang-Huo-Jiang,Ji-Chang-Jiu.Studies on chemical constituents in fruit of Alpinia oxyphylla[J].China Journal of Chinese Materia Medica,2009,34(8):990. | |
| Authors: | XU Dun-Ju Tan-Ning-Hu Ceng-An-Zhi Han-Hong-Jin Huang-Huo-Jiang Ji-Chang-Jiu |
| Affiliation: | 1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
| Abstract: | Objective: To study the chemical constituents in fruit of Alpinia oxyphylla and their cytotoxicities on cancer cell lines. Method: Compounds were isolated and purified by various column chromatographic methods. Their structures were determined by physico-chemical properties and spectral analyses. Compound cytotoxicity was assessed by the sulforhodamine B (SRB) assay. Result: Eight compounds were obtained from Me2CO-H2O (70%) extract of the fruit of A. oxyphylla and their structures were identified as: (9E)-humulene-2, 3; 6, 7-diepoxide (1), 3(12), 7(13), 9(E)-humulatriene-2, 6-diol (2), (-)-oplopanone (3), yakuchinone A (4), yakuchinone B (5), tectochrysin (6), isovanillin (7), (2E, 4E)-6-hydroxy-2, 6-dimethylhepta-2, 4-dienal (8), and the cytotoxicities of compounds 1, 3-5 on cancer cell lines, A549, HT-29 and SGC-7901, were also investigated. Conclusion: Compounds 1-3, 7, 8 were isolated for the first time from this genus and compounds 1, 3-5 exhibited no cytotoxicity against three cancer cell lines at a concentration of 10 mg·L-1. |
| Keywords: | Zingibereae Alpinia oxyphylla chemical constituents |
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