COX-2和VEGF表达及其与胆管癌的相关性研究

目的:探讨环氧合酶-2(COX-2)和血管内皮生长因子(VEGF)在胆管癌组织中的表达及其与胆管癌发生、发展及转移的关系。方法:运用免疫组织化学SP法研究COX-2和VEGF相关抗原在胆管癌组织中的表达。结果:43例胆管癌组织中COX-2和VEGF的表达阳性率分别为81.40%和76.74%,而二者在9例胆管结石病人的组织中均未见阳性表达;COX-2表达阳性率在胆管癌高、中分化组明显高于低分化组,转移阳性组明显高于转移阴性组,其差异均有显著性意义(P<0.05);VEGF的表达阳性率在胆管癌转移组明显高于未转移组,其差异有显著性意义(P<0.05);COX-2表达与VEGF表达呈正相关(r=0.727,P<0.01)。结论:COX-2和VEGF可能在胆管癌的发生、发展及转移过程中起着关键性作用,二者在血管生成过程中密切相关。

Correlation of Between Expressions of COX-2 and VEGF in Cholangiocarcinama

Objective: To investigate the expression of cyclooxygenase-2(COX-2) and vascular endothelial growth factor(VEGF) in human cholangiocarcinoma and their correlation with clinicobiological behavior.Methods: Expressions of COX-2 and VEGF in 43 cases of human cholangiocarcinoma were detected with immunohistochemistry of SP.Results:Expression rates of COX-2 and VEGF in cholangiocarcinoma were 81.40% and 76.74%,respectively.No expression of COX-2 and VEGF in caculus of bile duct was detected.The positive rate of COX-2 was significantly higher in well-differentiated and moderately-differentiated or metastatic tumors than in poorly-differentiated or non-metastatic tumors.The positive rate of VEGF was significantly higher in metastatic tumors than in non-metastatic tumors.Conclusion: COX-2 and VEGF may play a crucial role in tumorigenesis,tumor progression and metastasis in cholangiocarcimoma and both may have close relation in the angiogenesis.