种属特异性PCR法鉴别罐头食品中猪、牛、羊、鸡、鸭源性成分

建立适用于肉类罐头等长时间高温加工食品中猪、牛、羊、鸡、鸭5种动物源成分种属特异性PCR鉴别方法。通过使用猪、牛、羊、鸡、鸭的种属特异性引物,对5种动物的总DNA模板进行PCR扩增,得到分别为212、147、202、131、201 bp的扩增产物,将测序结果在美国国家生物技术信息中心(US National Center for Biotechnology Information,NCBI)进行BLAST比对确认实验的准确性,并对市售罐头样品进行检测。该方法5种动物引物种属特异性良好,对猪、牛、羊、鸭源性成分的检测灵敏度可达1%,对鸡源性成分的检测灵敏度为2.5%。在对市售肉类罐头样品的检测中,6.6%样品与标签标注结果不符。该方法操作简便,成本低,结果准确可靠,可广泛用于肉类罐头食品和长时间高温加工食品中猪、牛、羊、鸡、鸭5种动物源成分的鉴别,具有十分广泛的实际应用价值。

Species-specific PCR method to identify animal-derived ingredients of pork,beef,mutton,chicken and duck in canned food

A species-specific PCR identification method was established to identify five kinds of animal-derived components of pork,beef,mutton,chicken and duck in long-term high-temperature processed foods such as canned meat.The total DNA templates of five kinds of animals were amplified by PCR.Then the amplified products of 212 bp,147 bp,202 bp,131 bp,and 201 bp were obtained.Moreover,the sequencing results were verified by using the BLAST comparison on NCBI to confirm the accuracy of the experiment,and the commercially available canned samples were also been tested.In this method,the five animal introduced species have good specificity,the detection sensitivity for derived components of pork,beef,mutton and duck could reach 1%,and the detection sensitivity for chicken-derived components was 2.5%.After testing the commercially available samples,it was clear that in 6.6%of the samples,the actual composition did not match the label.This method was easy to operate,low in cost,accurate,reliable,which could be widely used in the identification of five animal-derived components of pigs,cattle,sheep,chickens and ducks in canned meat and long-term high-temperature processed foods.Above all,it had extensive value in applications.