| 应用光谱技术无损检测油菜叶片中乙酰乳酸合成酶 |
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| 引用本文： | 刘飞,方慧,张帆,金宗来,周伟军,何勇.应用光谱技术无损检测油菜叶片中乙酰乳酸合成酶[J].分析化学,2009,37(1). |
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| 作者姓名： | 刘飞 方慧 张帆 金宗来 周伟军 何勇 |
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| 作者单位： | 1. 浙江大学生物系统工程与食品科学学院,杭州,310029
2. 浙江大学农业与生物技术学院,杭州,310029 |
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| 基金项目： | 国家科技支撑项目，国家自然科学基金，国家高技术研究发展计划(863计划)，浙江省自然科学基金 |
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| 摘 要： | 应用可见/近红外光谱技术实现了油菜叶片中乙酰乳酸合成酶(ALS)的快速无损检测.对99个油菜样本进行光谱扫描,经过平滑、变量标准化、一阶求导等预处理后,应用偏最小二乘法(PLS)建立了ALS的预测模型.同时提取有效特征变量,作为反向传输人工神经网络(BPNN)和最小二乘-支持向量机(LS-SVM)的输入值,并建立相应的模型.用66个样本建模,33个样本验证.结果表明,LS-SVM模型能够获得最优的预测结果,预测集样本的相关系数(r)、预测标准差(RMSEP)和偏差(Bias)分别为0.998、 0.715和0.079,获得了满意的预测精度.结果表明,应用可见/近红外光谱技术结合LS-SVM检测油菜中乙酰乳酸合成酶是可行的,并能获得满意的预测精度,为进一步应用光谱技术进行油菜生长状况的大田监测奠定了基础.
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| 关 键 词： | 可见/近红外光谱 油菜 乙酰乳酸合成酶 偏最小二乘法 反向传输人工神经网络 最小二乘-支持向量机 |
Nondestructive Determination of Acetolactate Synthase in Oilseed Rape Leaves Using Visible and Near Infrared Spectroscopy |
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| LIU Fei,FANG Hui,ZHANG Fan,JIN Zong-Lai,ZHOU Wei-Jun,HE Yong.Nondestructive Determination of Acetolactate Synthase in Oilseed Rape Leaves Using Visible and Near Infrared Spectroscopy[J].Chinese Journal of Analytical Chemistry,2009,37(1). |
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| Authors: | LIU Fei FANG Hui ZHANG Fan JIN Zong-Lai ZHOU Wei-Jun HE Yong |
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| Affiliation: | College of Biosystems Engineering and Food Science;Zhejiang University;Hangzhou 310029;College of Agriculture and Biotechnology;Hangzhou 310029 |
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| Abstract: | Visible and near infrared(Vis/NIR) spectroscopy was applied for the fast and nondestructive determination of acetolactate synthase(ALS) in oilseed rape leaves.Ninety-nine samples were collected for Vis/NIR spectral scanning.Smoothing way of Savitzky-Golay with 9 segments,standard normal variate(SNV) and first derivative were used as preprocessing methods of spectral data before the calibration stage.Partial least squares(PLS) analysis was applied as calibration method as well as a way to extract the new eig... |
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| Keywords: | Visible and near infrared spectroscopy oilseed rape acetolactate synthase partial least squares analysis back propagation neural network least squares-support vector machine |
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