酪胺分子印迹聚合物的制备及识别特性研究

利用分子印迹技术制备用于酪胺快速检测的分子印迹聚合物.以酪胺为模版分子,甲基丙烯酸(MAA)为功能单体,偶氮二异丁腈(AIBN)为引发剂,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,在乙腈中沉淀聚合制备了酪胺分子印迹聚合物微球.通过紫外光谱法对酪胺与MAA的相互作用进行了分析,结果表明在研究的浓度范围内主客体主要存在形式为1个酪胺分子与1个MAA分子发生作用.对聚合物吸附动力学进行了初步研究,通过静态平衡吸附实验研究了聚合物微球对模板分子的结合能力,印迹聚合物微球在8h后逐渐达到吸附平衡.利用Langmuir数学模型对吸附特性进行了分析,Scatchard图显示印迹聚合物的最大吸附量Bmax=325.0μmol/g和解吸常数KD=0.577mmol/L.同时印迹聚合物的吸附选择性较好.此方法合成的印迹聚合物微球对酪胺有较好的结合性能,可应用于酪胺的分离检测.

Preparation and recognition properties of tyramine molecularly imprinted polymers

A polymer used for rapid determination of tyramine was prepared by molecular imprinting technology.The uniformly imprinted polymer microspheres were prepared by precipitation polymerization in acetonitrile using tyramine as template,methacrylic acid（MAA） as functional monomer,azobisisobutyronitrile（AIBN） as initiator and ethylene glycol dimethaerylate（EGDMA） as cross-linker,respectively.The UV spectrophotometry was used to demonstrate the interaction between tyramine and MAA.It was found that in this study one tyramine was entrapped by one MAA molecules,which was the main existing form of subject and object.The absorption kinetics of imprinted polymer were studied,as well as the adsorption efficiency by equilibrium adsorption experiments.The results showed that the imprinted polymer microspheres reached equilibrium gradually after 8 h and had higher adsorption efficiency than the non-imprinted polymer microspheres,which had the same chemical composition.The recognition properties were analyzed by Langmuir model.The Scatchard chart showed the apparent maximum binding capacity（Bmax） and dissociation content（KD） of the imprinted polymer were 325.0μmol/g and 0.577mmol/L,respectively.The absorption selectivity of imprinted polymer was nice.The tyramine imprinted polymer microspheres synthesized in this method had good performance and could be used for separation,enrichment and analysis of tyramine in foods.