郎罕细胞和淋巴细胞在贲门癌间质中的分布及其免疫学意义

目的探讨郎罕细胞（ＬＣ）和淋巴细胞在贲门癌组织中的分布及其免疫学意义，为判断病人预后提供理论根据。方法以ＬＳＡＢ免疫组化法，用Ｓ－１００蛋白抗体对正常贲门粘膜、单纯增生、不典型增生及癌组织进行染色，油镜下观察ＬＣ及淋巴细胞的分布及数量变化，将所得结果用秩和检验法进行统计分析。结果贲门粘膜单纯增生、不典型增生组织中ＬＣ的数量较癌组织中的少，正常粘膜组织内更少见。癌组织中ＬＣ的数量与其分化程度关系密切，同时在密集的区域常有淋巴细胞伴随。结论牌号癌组织内Ｓ－１００蛋白阳性ＬＣ及淋巴细胞的多少，可作为宿主抗肿瘤免疫反应的一个标志。

Distribution and immunological significance of Langerhans' cells and lymphocyte in interstitial tissue of cardia cancer

Objective To research the distribution andimmunological significance of Langerhans' cellsand lymphocytes in the interstitial tissue of cardialcareer and to provide scientific basic for evaluating the patients' prognosis.Methods Using anti-bovine S-100 protienantibodies by the way of LSAB immunohistochemical method, the immune stains wereperformed on normal cardiac mucosa, simplehyperplasia, dysplasia and carcinoma tissue.Thedistribution and number change of Langerhans'cells and lymphocytes were observed under an oillens. The results were analysised with rank sumtest.Results The number of Langerhans' cells intissue of simple and atypical hyperplasia was fewerthan that in cancer tissue, and the fewest of thatin normal cardiac mucosa. The number ofLangerhans' cells in cancer tissue was closelycorrelative to the degree of differentiation ofcardiac cancer, and the dense area ofLangerhans' cells was often accompanied bylymphocytes.Conclusion The number of S-100 protein positive Langerhans' cells and lymphocytes could be amarker for antineoplastic response of host.