| HPLC-ELSD法测定复方川贝母片中贝母素甲的含量 |
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| 引用本文： | 高光伟,张建业,冯向东.HPLC-ELSD法测定复方川贝母片中贝母素甲的含量[J].中国药师,2010,13(6):833-835. |
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| 作者姓名： | 高光伟 张建业 冯向东 |
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| 作者单位： | 1. 南阳市中心医院,河南南阳,473000
2. 南阳市食品药品检验所 |
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| 摘 要： | 目的：建立复方川贝母片中贝母素甲的含量测定方法.方法：用Agilent-ODS C18（250mm×4.6mm,5μg）枉,流动相为乙腈-水-二乙胺（70：30：0.3） ELSD漂移管温度70℃ 氮气流速2.0L·min-1 柱温35℃.结果：贝母素甲与相邻峰得到很好分离,进样量在0.165～0.990 μg时线性关系良好（r=0.999 2） 平均加样回收率为98.52%（RSD=2.56%）.结论：本法结果准确,便于操作,可作为复方川贝母片的质量控制方法之一.
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| 关 键 词： | HPLC—ELSD法 复方川贝母片 川贝母 贝母素甲 |
Determination of Peimine in Compound Fritillaria Tablets by HPLC-ELSD |
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| Gao Guangwei,Zhang Jianye,Feng Xiangdong.Determination of Peimine in Compound Fritillaria Tablets by HPLC-ELSD[J].China Pharmacist,2010,13(6):833-835. |
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| Authors: | Gao Guangwei Zhang Jianye Feng Xiangdong |
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| Affiliation: | ( Nanyang Institute for Food and Drug Contral) |
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| Abstract: | Objective： To establish the method of determining peimine in compound fritillaria tablets. Method： HPLC-ELSD was used to determine peimine in compound fritillaria tablets. The chromatographic analysis were carried on Agilent-ODS C18（250 mm ×4. 6 mm,5 μm） column, The mobile phase was acetonitrile- water -diethylamine （70： 30： 0. 3 ） at 35 ~C. Drift tube temperature was 70 ＇12, gas flow rate was 2. 0 L. min-1. Result： Peimine were separated well. The calibration curves was linear within the ranges of 0. 165- 0. 990 μg （r =0. 999 2） . The average recovery was 98. 52% （RSD =2. 56% ）. Conclusion： The method is simple and rapid,and is suitable for quality control of compound fritillaria tablets. |
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| Keywords: | HPLC-ELSD Compound fritillaria tablets FritiUariae cirrhosae bulbus Peimine |
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