酢浆草体外抗炎抗氧化检测方法的建立和优化

目的优化和建立酢浆草体外抗炎抗氧化活性的检测方法。方法建立抗氧化、蛋白酶活性抑制和蛋白变性抑制活性的检测方法。比较醇提水沉法和水提醇沉法、不同保存条件下药物的活性。结果优化检测方法后,不同浓度酢浆草样品的检测结果具有高度相关性,表明检测方法高度稳定且准确可靠。经醇提水沉法和水提醇沉法后,酢浆草的抗氧化活性为100%,蛋白变性抑制活性为90%,酶活性抑制活性为50%,两者出峰位置基本一致。醇提水沉法更易快速获得澄清的提取溶液。室温和4℃冷藏时,蛋白变性抑制成分第3天时均下降40%,第7天时下降至无活性;酶活性抑制成分在冷藏时下降比室温条件慢,在第7天时分别下降30%和60%;抗氧化成分在2种保存条件下均有较高活性。结论所建立的方法可用于体外检测酢浆草的抗炎活性。

Establishment and Optimization of in Vitro Anti-Inflammatory and Antioxidant Detection Method of Oxalidaceae

Objective To establish and optimize the in vitro anti-inflammatory and antioxidant detection method of Oxalidaceae. Methods The detection method of inhibitory activities of antioxidant,protease activity and protein denaturation were established. The activities of the drug were compared between alcohol extraction and water precipitation method,water extraction and alcohol precipitation method,and under different storage conditions. Results After optimizing the detection method,the detection results of different concentrations of Oxalidaceae samples had high correlation,indicating that the detection method was highly stable,accurate and reliable. After the alcohol extraction and water precipitation method,the antioxidant activity of the Oxalidaceae was 100%,the protein denaturation inhibitory activity was 90%,and the enzyme activity inhibitory activity was 50%. The peak positions of the two were basically the same. The alcohol extraction and water precipitation method made it easier to quickly obtain clear extraction solution. When stored at room temperature and refrigerated at 4 ℃,the protein denaturation inhibition component was decreased by 40% at day 3 and decreased to inactivity at day 7. Enzyme activity inhibitors decreased more slowly when refrigerated than at room temperature and decreased by 30% and 60% respectively at day 7. Antioxidant components were highly active under both storage conditions. Conclusion The established method can be used as the in vitro anti-inflammatory and antioxidant detection method for Oxalidaceae.