阿托伐他汀对骨髓来源的成骨细胞中OPG mRNA/RANKL mRNA水平的影响

目的:观察阿托伐他汀对体外培养骨髓基质细胞来源的成骨细胞中OPGmRNA/RANKLmRNA表达的影响。方法:小鼠骨髓基质细胞在成骨条件培养基中传代培养,加入不同浓度的阿托伐他汀。通过半定量RT鄄PCR的方法,比较不同浓度药物影响下OPGmRNA和RANKLmRNA表达的变化,评判阿托伐他汀对这两种蛋白表达的影响作用。结果:半定量RT鄄PCR的观察显示在传代培养7天时各组均有OPGmRNA的转录,随着药物浓度的增加,mRNA转录水平逐渐增加,10-6mol/L组和10-7mol/L组明显高于对照组(P<0.05)。RANKLmRNA水平随着药物浓度的增加呈现出下降的现象,10-6mol/L组和10-7mol/L组明显低于对照组。结论:在小鼠骨髓基质细胞来源的成骨细胞中,阿托伐他汀促进了成骨细胞OPG的表达,同时能抑制细胞RANKL的表达。

The effect of atorvastatin on the expression of OPG mRNA and RANKL mRNA of osteoblasts in vitro

Objective: To observe the effect of atorvastatin on the expression o f osteoprotegerin (OPG) mRNA and Receptor Activator of Nuclear Factor-kB Ligand (RANKL) mRNA of osteoblasts derived from mice bone marrow stromal cells. Methods : Osteoblasts derived from mice femurs bone marrow stromal cells of were culture d without or with 10-6-10-8 Matorvastatin medium in subculture. After 7 days, th e expressions of OPG mRNA and RANKL mRNA were obtained with semi-quantative RT-P CR. Results: Semi-quantative RT-PCR examination revealed OPG mRNA expression in each group after 7 days subculture. Atorvastatin promoted OPG mRNA expression si gnificantly in 10-6 mol/L groups and 10-7 mol/L groups, compared with the contro l group (P < 0.05). Atorvastatin inhibited osteoblasts RANKL mRNA expression in a dose-dependent manner, this effect was significantly in 10-6 mol/L group and 1 0-7 mol/L group, compared with the control group (P < 0.05). Conclusion: Atorvas tatin promotes bone stromal derived osteoblasts OPG expression, while it inhibit s RANKL expression in the same cells.