| 微生物发酵合成15N-L-脯氨酸 |
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| 引用本文： | 侯静华,孙建春. 微生物发酵合成15N-L-脯氨酸[J]. 食品与生物技术学报, 2003, 22(6): 42-45 |
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| 作者姓名： | 侯静华 孙建春 |
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| 作者单位： | 上海化工研究院新型材料研究所,上海,200062 |
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| 摘 要： | 采用含有稳定同位素15N 氯化铵为主要氮源的专用发酵培养基配方和相应的提取精制条件,采用微生物直接发酵方法研制15N L 脯氨酸高丰度产品.结果显示,每克15N 氯化铵实际可得0.376g15N L 脯氨酸,提取精制得率最高达65.76%,产品15N丰度达98.28%,比原料下降1.23%.
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| 关 键 词： | 15N-L-脯氨酸 生物合成 离子交换 提取 |
| 文章编号： | 1009-038X(2003)06-0042-04 |
| 修稿时间： | 2003-06-05 |
Biosynthesis of 15N-L-Proline by Fermentation |
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| HOU Jing-hu,SUN Jian-chun. Biosynthesis of 15N-L-Proline by Fermentation[J]. Journal of Food Science and Biotechnology, 2003, 22(6): 42-45 |
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| Authors: | HOU Jing-hu SUN Jian-chun |
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| Abstract: | ()~( 15)N-L-Proline was prepared by fermentation in a specific nutrient medium containing (()~(15)N-ammonium) chloride and growth stimulants, and with subsequent isolation and purification of ()~(15)N-L-Proline from fermentation liquid. 0.376 gram ()~(15)N-L-Proline was produced for one gram of ()~(15)N-ammonium chloride. The best purification yield reached 65.76 %. The enrichment of ()~(15)N-L-Proline was 98.28%, and that is 1.23% lower than ()~(15)N-ammonium Chloride. |
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| Keywords: | ~(15)N-L-Proline biosynthesis ion exchange isolation |
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