抗丁型肝炎病毒（HDV）抗体酶联免疫测定（ELISA）方法的建立与应用

ESTABLISHMENT AND APPLICATION OF ELISA FOR DETECTION OF ANTI-HDV ANTIBODY

Anti-HDV ELISA method was established with HDAg expressed in E.coli, purified anti-HDV IgG from human sera, and purified anti-HDV antibody labelled with HRP. This method was used to test anti-HDV antibody in 53 sera collected from HBsAg positive patients and HBsAg carriers. 21 cases were positive. In order to standardize this assay,these samples were also tested by Abbott anti-delta-EIA kit. The results showed that the 100% agreement.The ELISA titer of antibody in one positive serum was 1:80 000 by our ELISA reagent, but 1:10 000 by Abbott EIA kit.This method was used to detect anti-HDV antibody in sera of 1155 HBsAg positive patients and carriers from several provinces and areas of China. The positive rate correspond well with that obtained with Abbott kit. There was no false positive. Since our reagent has high specificity, good sensitivity, is cheap and convenient, it can substitute Abbott kit for clinical daignosis of hepatitis virus infection, epidemiological research and other purposes.