油气田土壤样品中可培养丁烷氧化菌多样性研究

分别采用丁烷或丁醇选择培养法,对采自四川省达州市普光气田和湖北省江汉油田的土壤样品中的丁烷氧化菌进行了分离、纯化、16S rRNA基因序列测定及系统发育研究,探讨不同油田不同环境中烃氧化菌数量、系统发育地位以及种群多样性的差异.结果表明,初步分离获得共25株丁烷氧化菌,分属于厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)和变形菌门(Proteobacteria).2个油田样品分离出的丁烷氧化菌群落结构简单,均包括芽孢杆菌(Bacillus)、假单胞菌(Pseudomonas)、红球菌(Rhodococcus)和节杆菌(Arthrobacter)4个属的细菌,苍白杆菌属(Ochrobactrum)和分支杆菌属(Mycobacterium)的细菌分离自普光气田.普光气田分离出的丁烷氧化细菌数量和多样性略高于江汉油田.

Diversity of Culturable Butane-oxidizing Bacteria in Oil and Gas Field Soil

Butane-oxidizing bacteria in soil sample sites from Puguang gas field in Sichuan province and Jianghan oil field in Hubei province were isolated and 16S rRNA gene sequence and phylogenetic analysis were applied. The differences of number and phylogenetic position and population diversity of hydrocarbon-oxidizing bacteria in different environment were investigated. The results show that 25 strains of butane-oxidizing bacteria were isolated. Based on sequencing of 16S rRNA gene, the species of bacteria in two samples are classified into 3 phyla including Firmicutes, Actinobacteria and Proteobacteria. The community structure of butane-oxidizing bacteria isolated from two oil samples is simple, both of them contain 4 genus including Bacillus, Pseudomonas, Rhodococcus and Arthrobacter. Strains in the genus of Ochrobactrum and Mycobacterium were only isolated from Puguang gas field. The number and population diversity of butane-oxidizing bacteria in Puguang gas field was more than those in Jianghan oil field.