| 固相萃取-分光光度法快速检测腐竹中的碱性橙 |
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| 引用本文： | 李小燕,仝海娟,石展望,罗少芹,秦玉凤,李梅.固相萃取-分光光度法快速检测腐竹中的碱性橙[J].食品工业科技,2011(11):447-450. |
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| 作者姓名： | 李小燕 仝海娟 石展望 罗少芹 秦玉凤 李梅 |
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| 作者单位： | 1. 广西民族大学化学与生态工程学院,广西南宁530006／广西林产化学品开发与应用重点实验室,广西南宁530006
2. 广西民族大学化学与生态工程学院,广西南宁,530006 |
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| 基金项目： | 广西自然科学基金项目(2010GXNSFA013047); 广西教育厅科研项目(200911LX84) |
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| 摘 要： | 目的：建立固相萃取净化富集,分光光度法快速检测腐竹中禁用色素碱性橙的方法。方法：样品经甲醇超声提取,C18固相萃取柱萃取,以20%甲醇溶液（V/V）为清洗剂净化,80%甲醇溶液（V/V）为洗脱剂洗脱富集后,用分光光度法进行测定。结果：碱性橙在2.0～10.0μg/mL浓度范围呈现良好的线性关系,相关系数R=0.9955;在2.0～8.0μg/mL添加水平时回收率范围为102.24%～84.15%,重复性实验平均回收率为98.45%,相对标准偏差为1.87%（n=5）。结论：该法灵敏度高、重复性好,操作简便快速,结果准确,适用于腐竹中碱性橙的快速检测。
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| 关 键 词： | 固相萃取 分光光度法 碱性橙 腐竹 |
Rapid determination of basic orange in dried beancurd sticks by solid phase extraction-spectrophotometry |
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| LI Xiao-yan,TONG Hai-juan,SHI Zhan-wang,LUO Shao-qin,QIN Yu-feng,LI Mei.Rapid determination of basic orange in dried beancurd sticks by solid phase extraction-spectrophotometry[J].Science and Technology of Food Industry,2011(11):447-450. |
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| Authors: | LI Xiao-yan TONG Hai-juan SHI Zhan-wang LUO Shao-qin QIN Yu-feng LI Mei |
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| Affiliation: | LI Xiao-yan1,2,TONG Hai-juan1,SHI Zhan-wang1,LUO Shao-qin1,QIN Yu-feng1,LI Mei1(1.College of Chemistry and Ecological Engineering,Guangxi University for Nationalities,Nanning 530006,China,2.Key Laboratory of Development & Application of Forest Chemicals of Guangxi,China) |
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| Abstract: | Objective:A novel method based on solid phase extraction followed by Spectrophotometry was developed for the analysis of prohibited colorant basic orange in dried beancurd sticks.Method:Basic orange in dried beancurd sticks was extracted with SIPORE SPE-C18SPE column,purified by 20% methanol aqueous solution(V/V),eluted by 80% methanol aqueous solution(V/V)and detected by Spectrophotometry with mensurated at the maximum absorption wavelength 446nm.Results:The linear correlation coefficient was 0.9955 over t... |
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| Keywords: | solid phase extraction spectrophotometry basic orange dried beancurd sticks |
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