日本血吸虫虫卵与旋毛虫肌蚴间相似抗原的研究

目的 :分析日本血吸虫虫卵与旋毛虫肌蚴间共同抗原的相似程度。方法 :应用 EITB和 ELISA技术对此 2种寄生虫可溶性抗原进行了血清学交叉反应分析。结果 :EITB显示 :在Ts ML A中 ,4 4、 53、 60、 64、 66/ 70 k Da和约 10 0 k Da的抗原为 Sj IRS识别 ,而 31、 4 2、 4 6、 51/53和 58/ 60 k Da成分为 α- Sj EARS识别 ;在 Sj EA中 ,4 6、58和 64k Da抗原为 Ts IRS识别 ,58、60、64和 70 k Da成分为α- Ts MLARS识别。EL ISA显示 :在 Sj IRS中抗 Ts抗体滴度显著高于在Ts IRS中所含抗血吸虫卵的抗体滴度。结论 :日本血吸虫卵与旋毛虫肌蚴间存有较多的共同抗原决定簇 ,但两者间的相似程度有明显差异。

NTIGENIC SIMILARITY BETWEEN SCHISTOSOMA JAPONICUM EGG AND TRICHINELLA SPIRALIS MUSCLE LARVA ANTIGEN

AIM:To analyse the antigenic similarity between Schistosoma japonicum egg and Trichinella spiralis muscle larva(TsMLA). METHODS:Soluble egg antigen of S.japonicum (Sj EA) and soluble muscle larva antigen of T.spiralis (TsMLA) were analysed by EITB and ELISA techniques. RESULTS:EITB showed that:in TsMLA, the antigens of 44, 53, 60, 64, 66/70 and 100 kDa were recognized by SjIRS, while 31, 42, 46, 51/53 and 58/60 kDa antigens byα- SjEARS; in SjEA, the antigens of 46, 58 and 64 kDa were recognized by TsIRS, while 58, 60, 64 and 70 kDa proteins by α-T sMLARS. ELISA showed that the levels of antibodies against T. spiralis larva antigens in SjIRS were higher than those of antibodies against S. japonicum egg antigens in TsIRS. CONCLUSION: SjEA and TsMLA shared common antigens mainly among 44-70 kDa, but there were also differences in antigenic similarity between both S.japonicum egg and T.spiralis muscle larva.