核素标记自体骨髓单个核细胞监测心脏干细胞移植后存活情况

Objective To observe in vivo stem cell distribution and viability after transplantation by noninvasive imaging of ~(18)F-fluorodeoxyglucose( ~(18)F-FDG)labeled autologous mononuclear bone marrow cells.Methods Myocardial infarction was established in 8 swine by ligating left anterior descending coronary artery after anesthesia. Bone marrow (20 ml) was drawn through ileum. After isolation, mononuclear bone marrow cells were labeled by radionuclide ~(18)F-FDG and intramyocardially injected into infarction region. Whole body planar scan and myocardial tomography scan were performed immediately, 1 h, 2 h, and 3 h post stem cell injection. Viability and stability of radionuclide labeled stem cells were determined at 3 h post labeling in vitro. Results The labeling efficiency was (67 ± 14)%. Mean dose of radioactive in marrow cells was (32 ±7)MBq. Trypan blue staining showed in vitro viability was (95 ±3)% at 3 h post labeling.After intramyocardial injection, labeled mononuclear bone marrow cell retention rate in infarction region was (83 ± 6) %, (49 ± 8 ) %, ( 32 ± 6) % and (24 ± 5 ) % immediately, 1 h, 2 h, and 3 h post injection, respectively. Conclusions Distribution and viability of stem cell after cardiac transplantation could be effective monitored by ~(18)F-FDG labeled autologous mononuclear bone marrow cell technique in acute stage in this model.

Feasibility of in vivo isotope labeled autologous mononuclear bone marrow cells monitoring post cardiac transplantation in a swine model of myocardial infarction