| 超微粉碎稻谷糠壳发酵生产燃料丁醇的研究 |
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| 引用本文： | 张良,袁永俊,杨攀.超微粉碎稻谷糠壳发酵生产燃料丁醇的研究[J].西华大学学报(自然科学版),2012(6):92-97. |
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| 作者姓名： | 张良 袁永俊 杨攀 |
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| 作者单位： | 西华大学生物工程学院食品生物技术四川省高校重点实验室 |
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| 基金项目： | 国家教育部“春晖计划”项目(Z2011095);四川省教育厅青年基金项目(12ZA167);西华大学重点科研基金项目(XZD1120538) |
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| 摘 要： | 以超微粉碎后的稻谷糠壳为原料，以丙酮丁醇梭菌为发酵菌株，考察了不同硫酸浓度和酸水解时间对发酵生产丙酮、丁醇、乙醇（ABE）总溶剂浓度的影响。同时进一步研究其酸水解液不同脱毒方式，添加碳源和营养物质对ABE总溶剂产量的影响。结果表明：体积分数为1．5％的硫酸121℃酸水解30min，利用Ca（OH）：过中和酸水解液脱毒，发酵78h最终ABE总溶剂量为14．62g/L，较未处理提高30．10％，残糖质量浓度为8．46g／L，较未处理下降47．8％；超微粉碎后稻谷糠壳酸水解液中的营养成分已能满足丙酮丁醇梭菌发酵生产ABE溶剂所需，添加葡萄糖碳源和其他营养成分并不能显著提高ABE总溶剂产量和缩短发酵时间。
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| 关 键 词： | 稻谷糠壳 丁醇 ABE发酵 酸水解 |
Application of Superfine Grinding Rice Hull Acid Hydrolysis in Acetone-Butanol-Ethanol(ABE) Fermentation |
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| ZHANG Liang,YUAN Yong-jun,YANG Pan.Application of Superfine Grinding Rice Hull Acid Hydrolysis in Acetone-Butanol-Ethanol(ABE) Fermentation[J].Journal of Xihua University:Natural Science Edition,2012(6):92-97. |
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| Authors: | ZHANG Liang YUAN Yong-jun YANG Pan |
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| Affiliation: | Key Lab Food Biotechnology of Sicaauan Province,School of Bioengineering,Xihua University,Chengdu 610039 China |
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| Abstract: | The process for producing ABE by fermentation from Superfine grinding rice hull acid hydrolysis was studied. Based on the ABE concentration, fermentation time, reduce sugar concentration and initial sugar concentration, different detoxification methods and acid hydrolysis rate were investigated. The optimized conditions for the production were 1.5 % （V/V） of Sulfuric acid hydrolysis for 30 min at 121℃, then neutralization by Ca（ OH）2 for detoxification. After 78h, 14.62 % （V/V） of ABE was produced, and remained sugar was only 8.46 g/L. Since it provided the necessary carbon source and nutrition for ABE fermentation, there was no need to supplement these additionally. |
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| Keywords: | rice hull butanol ABE fermentation acid hydrolysis |
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