| 人食道癌细胞系CaEs—17放射敏感性与H—ras的扩增 |
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| 引用本文： | 王顺宝,郭蕾.人食道癌细胞系CaEs—17放射敏感性与H—ras的扩增[J].辐射研究与辐射工艺学报,1997,15(1):60-62. |
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| 作者姓名： | 王顺宝 郭蕾 |
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| 作者单位： | 中国医学科学院肿瘤研究所中国协和医科大学肿瘤医院放射生物室!北京100021 |
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| 摘 要： | 从我国自行建株的人食道癌细胞系ＣａＥｓ－１７野生型中分离出两株放射抗拒的亚系克隆７和克隆１０，其ＳＦ２值分别为０．４１、０．５８和０．６１。存活曲线的分析表明，与野生型比较，克隆了７及克隆１０的Ｄ０值差异不大，但ＤＱ与Ｎ值有较大的差别。野生型细胞系ＤＱ为１．０７Ｇｙ，而克隆７的ＤＱ为１．８５Ｇｙ，克隆的１０的ＤＱ值为２．０４Ｇｙ；野生型细胞的Ｎ值为１．９６，克隆７亚系的Ｎ值为３．０７，克隆１０亚系
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| 关 键 词： | 放射敏感性 食道癌细胞系 H－ras |
HETEROGENEITY IN RADIOSENSITIVITY WITHIN ESOPHAGEAL CELL LINE CaEs-17 AND AMPLIFICATION OF H-ras GENE |
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| Wang Shunbao, Guo Lei, Niu Wenzhe.HETEROGENEITY IN RADIOSENSITIVITY WITHIN ESOPHAGEAL CELL LINE CaEs-17 AND AMPLIFICATION OF H-ras GENE[J].Journal of Radiation Research and Radiation Processing,1997,15(1):60-62. |
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| Authors: | Wang Shunbao Guo Lei Niu Wenzhe |
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| Abstract: | Ten clones were picked from cultured colonies of CaEs-17 cell line and developed to a subcell line. The values of SF2 were measured for each subcell line.Two radioresistant subcell lines, clone 7 and clone 10 were established.According to survival curve assay,for wild type, the value of Do was 1. 57 Gy, DQ was 1.07Gy,N was 1. 96, SF, was 0. 41. For clone 7,the value of D, was 1.64 Gy, DQ was 1.85Gy, N was 3.07, SF2 was 0.53. For clone 10, the value of Do was 1.58 Gy,DQ was 2.04 Gy, N was 3.63, SF2 was 0.61. Clone 7 and clone 10 have much higher values of DQ and N than those of wild type. there was amplification of H-ras gene in clone 10 after 2 Gy irradiation. The amplification of H-ras gene in clone 10 after 2 Gy irradiation might be involved in hetero geneity of CaEs-17 cell line. |
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| Keywords: | Radiosensitivity H-ras gene Survival curve Human esophageal carcinoma cell line |
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