复方硫磺软肝丸抗慢性肝纤维化作用研究

目的探讨复方硫磺软肝丸对改良方法所致大鼠慢性肝纤维化模型的保肝及抗纤维化作用。方法采用配方饲料加腹腔注射CCl4橄榄油溶液的改良方法复制大鼠慢性肝纤维化模型。造模结束后，灌胃给药，每天2次,连续给药1 个月。末次给药后禁食12 h,留取大鼠血清及肝组织，检测血清中转氨酶（ALT、AST）指标、血清肝纤维化（HA、LN、Col-Ⅳ）指 标的变化，观察肝组织病理切片的变化。结果模型组大鼠有明显的肝损伤及肝纤维化病变;各给药组肝功能和肝纤维化程 度均有不同程度的改善，病理组织学检查结果表明其肝脏病变程度较模型组明显减轻（P〈0.05）。结论复方硫磺软肝丸 对慢性肝纤维化大鼠有明显的保肝及抗肝纤维化作用。

Effect of Compound Liuhuangruangan Pills on Liver Fibrosis in Rats

Objective To investigate the hepatoprotective and antifibrotic effect of compound Liuhuangruangan pills on chronic hepatic fibrosis induced by improved methods in rats. Methods Hepatic fibrosis models were induced by hypodermic injection of carbon tetrachloride olive oil solution and high-fat diet. After establishment of fibrosis models,rats were ig given experimental drugs,positive drugs or the vehicle for 1 month, twice a day. Rats were then sacrificed after the last administration and their serum and livers were collected. The levels of ALT, AST and HA, LN, Col-IV were determined and pathological changes in liver tissues were observed. Results The rats in the model group suffered obvious hepatic damage and chronic fibrosis. Liver function and hepat-ic fibrosis were improved in drug groups. The pathological observation of the liver showed that the degree of hepatic fibrosis was remarkably alleviated in drug groups. Conclusion The compound Liuhuangruangan pills play an im-portant role in preventing chronic fibrosis of the rat liver.