电子束辐射对铜绿微囊藻蛋白质及抗氧化体系酶活性的影响

铜绿微囊藻以其毒素和产生的嗅味威胁人类健康和安全，且因大量生长和难去除性给水处理带来困难。本文以电子加速器产生的电子束对铜绿微囊藻培养液（对数生长期）分别进行1、2、3、4和5 kGy剂量的辐射，通过考察藻细胞蛋白质、抗氧化体系酶活性及光合速率的变化，研究辐射对铜绿微囊藻生命特征及机能产生的影响。结果表明：1 kGy的辐射对藻细胞可溶性蛋白质及POD、SOD活性的影响较小；而2～5 kGy的辐射明显降低了蛋白质含量，并破坏了藻细胞的抗氧化系统，光合速率也因辐射明显降低，使铜绿微囊藻短时间内失活。表明一定剂量的电子束辐射能有效控制藻细胞生长，并影响其重要生命特征。

Effect of Irradiation of Electron Beam on Protein and Antioxidized Enzyme Activity of Microcystis aeruginosa

Microcystis aeruginosa often threatens human health and safety for its microcystin and bad smell. Its large number and hardness of removal are difficulty for water treatment. In this study, electron beam generated by an accelerator was applied to irradiate Microcystis aeruginosa by dose of 1, 2, 3, 4 and 5 kGy. The effect of irradiation on Microcystis aeruginosa characteristic and mechanism was studied by surveying the changing of protein, enzyme activity and photosynthesis rate. The data show that irradiation of 1 kGy has little effect on dissoluble protein, POD and SOD activity. Irradiation of 2-5 kGy can decrease protein content and destroy the antioxidant system, also the photosynthesis rate decreases obviously, which makes Microcystis aeruginosa lose activity in short time. The result proves that a certain dose of electron beam irradiation can control algae growth and affect its life characteristic efficiently.