浙江省鸭、鸡、鹅群感染坦布苏病毒的血清学调查与分析

为分析坦布苏病毒（TMUV）在鸭、鸡、鹅群中的感染情况，开展了血清流行病学调查。2010-2014年间，在浙江省11个地市采集鸡、鸭、鹅血清样品4 989份，采用ELISA方法检测TMUV抗体水平。结果表明：鸭、鹅、鸡的抗体阳性率分别为27.33%，6.94%，1.11%。按年度分析，在2010-2011年鸭TMUV引起的产蛋下降疫情流行期间，鸭感染群的TMUV抗体阳性率为54.83%，鹅、鸡未采样检测；2012年鸭、鹅TMUV感染群的抗体阳性率分别为33.88%，20.00%，鸡未检测到抗体阳性群；2013年鸭、鹅、鸡TMUV感染群的抗体阳性率分别为49.68%，10.00%，28.00%；2014年鸭、鹅TMUV感染群的抗体阳性率分别为39.19%，6.67%，鸡未检测到抗体阳性群。总体来看，鸭群在2010-2011年TMUV流行期间的抗体水平最高，表明鸭群是TMUV感染的主要禽种。

Seroepidemiological investigation and analysis on duck Tembusu virus antibody level in Zhejiang Province

In the present study， seroepidemiological investigation on duck Tembusu virus （TMUV） antibody level was carried out. A total of 4 989 sera samples were collected from duck， goose and chicken during 2010-2014 in Zhejiang Province. The TMUV antibody levels of collected samples were detected by ELISA method. It was shown that the positive rates of TMUV antibody in duck， goose and chicken samples were 27.33%， 694% and 111%， respectively. By yearly analysis， TMUV antibody positive rate was 54.83% in the infected duck flocks in 2010-2011 during epidemic period. Goose and chicken sera samples were not collected in that period. TMUV positive rates in the infected duck and goose flock were 3388% and 20.00%， respectively， in 2012， and positive serum sample was not found in chicken flocks. TMUV positive rates in the infected duck， goose and chicken flock were 49.68%， 10.00% and 28.00%， respectively in 2013. TMUV positive rates in the infected duck and goose flock were 39.19% and 6.67%， respectively， in 2014， and no positive serum sample was found in the chicken flocks. In conclusion， the highest TMUV antibody level was found in the year of 2010-2011 during the epidemic period， and duck was the main TMUV infected poultry.