|
|||||
|
|
| 中国明对虾Kazal型丝氨酸蛋白酶抑制剂基因(Fc-Kazal)的重组表达及活性分析 | |
| 引用本文： | 黄明,刘逸尘,张亦陈,孙妍,孙金生. 中国明对虾Kazal型丝氨酸蛋白酶抑制剂基因(Fc-Kazal)的重组表达及活性分析[J]. 水产学报, 2011, 35(9): 1310-1319. DOI: 10.3724/SP.J.1231.2011.17473 |
| 作者姓名： | 黄明 刘逸尘 张亦陈 孙妍 孙金生 |
| 作者单位： | 1. 天津师范大学生命科学学院,天津300387;天津市细胞遗传与分子调控重点实验室,天津300387 2. 天津市细胞遗传与分子调控重点实验室,天津,300387 3. 天津师范大学生命科学学院,天津300387;天津市细胞遗传与分子调控重点实验室,天津300387;天津市水产养殖病害防治中心,天津300221 |
| 基金项目： | 国家自然科学基金项目(30871907,30901119,30901094);天津市科委(10JCZDJC18200,10JCYBJC09200,09JCYBJC15000) |
| 摘 要： | Kazal型丝氨酸蛋白酶抑制剂可以通过精确调控丝氨酸蛋白酶的活力,在生物体的防御应答等众多生物过程中发挥重要作用。以前期克隆的中国明对虾Kazal型丝氨酸蛋白酶抑制剂基因(Fc-Kazal, GenBank注册号为DQ318856)为基础,对其功能结构域进行序列比对和进化分析;组织表达分析结果表明,该基因在血细胞、鳃和淋巴器官等组织中高水平表达,而在眼柄、神经和肌肉中无表达;利用原核表达系统对该基因成熟肽区域成功进行了重组表达,纯化后的目的蛋白最终得率为0.4 g/L培养液;活性分析结果显示,复性后的rFc-Kazal对鳗弧菌、金黄色葡萄球菌、杀鲑气单胞菌、苏云金芽孢杆菌有明显的抑菌作用。 |
| 关 键 词： | 中国明对虾 Kazal型丝氨酸蛋白酶抑制剂 先天免疫 重组表达 抑菌活性 |
| 收稿时间： | 2011-04-09 |
| 修稿时间： | 2011-06-24 |
Recombinant expression and bioactivity assay of Kazal type serine proteinase inhibitor(Fc-Kazal)from Fenneropenaeus chinensis |
|
| HUANG Ming,LIU Yi-chen,ZHANG Yi-chen,SUN Yan and SUN Jin-sheng. Recombinant expression and bioactivity assay of Kazal type serine proteinase inhibitor(Fc-Kazal)from Fenneropenaeus chinensis[J]. Journal of Fisheries of China, 2011, 35(9): 1310-1319. DOI: 10.3724/SP.J.1231.2011.17473 | |
| Authors: | HUANG Ming LIU Yi-chen ZHANG Yi-chen SUN Yan SUN Jin-sheng |
| Affiliation: | College of Life Science,Tianjin Normal University;Tianjin Key Laboratory of Cyto-Genetical & Molecular Regulation,College of Life Science,Tianjin Normal University;Tianjin Key Laboratory of Cyto-Genetical & Molecular Regulation,College of Life Science,Tianjin Normal University;Tianjin Key Laboratory of Cyto-Genetical & Molecular Regulation,Tianjin Aquaculture Disease Prevention & Treatment Center,College of Life Science,Tianjin Normal University;Tianjin Key Laboratory of Cyto-Genetical & Molecular Regulation;Tianjin Aquaculture Disease Prevention & Treatment Center |
| Abstract: | Chinese shrimp(Fenneropenaeus chinensis)is one of the most important aquaculture animals in China.The studies on the innate immune responses of shrimp,especially on immune defense against the main crustacean pathogens,will provide more knowledge of shrimp immunity to prevent infectious diseases.Arthropod innate defence responses[e.g.prophenoloxidase(proPO)activation and Toll pathway initiation]and many other biological processes are mediated by serine proteinase(SP)cascades.If the activity of SPs is out of ... |
| Keywords: | Fenneropenaeus chinensis Kazal serine proteinase inhibitor innate immunity recombinant expression bacteriostatic activity |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《水产学报》浏览原始摘要信息 | |
| 点击此处可从《水产学报》下载全文 | |
