基于丙烯酰胺的黄曲霉毒素分子印迹聚合物的制备及性能研究

目的 制备基于丙烯酰胺的黄曲霉毒素分子印迹聚合物(molecularly imprinted polymer, MIPs)并研究其吸附性能。方法 以黄曲霉毒素的结构类似物5,7-二甲氧基香豆素(5,7-dimethoxy coumarin, DMC)为假模板分子,以丙烯酸酯(acrylate,AAM)为功能单体,采用溶胶凝胶表面印迹技术制备了新型核壳型黄曲霉毒素SiO₂包裹Fe₃O₄分子印迹聚合物(Fe₃O₄@SiO₂ MIPs),并对其进行透射电镜、傅里叶变换红外、X射线衍射、振动磁强表征以及吸附动力学实验和吸附结合实验,研究Fe₃O₄@SiO₂ MIPs的吸附性能。结果 所制备的Fe₃O₄@SiO₂ MIPs具有优良的选择性、高的吸附容量、快速的吸附动力学,在60 min达到最大吸附容量4.289 mg/g,印迹因子为1....

Study of preparation and properties of aflatoxin molecularly imprinted polymer based on acrylamide

Objective To prepare the aflatoxin molecularly imprinted polymer (MIPs) based on acrylamide, and study its adsorption properties. Methods Aflatoxins structural analog 5,7-dimethoxy coumarin (DMC) was used as a pseudo-template molecule, and acrylate (AAM) was used as a functional monomer, a novel core-shell aflatoxins silicon dioxide ferrosoferric oxide molecularly imprinted polymer (Fe3O4@SiO2 MIPs) was prepared by sol-gel surface imprinting technique, and subjected to transmission electron microscopy, Fourier transform infrared, X-ray diffraction, vibrational magnetic intensity characterization as well as adsorption kinetics experiments and adsorption binding experiments to investigate the adsorption properties of Fe3O4@SiO2 MIPs. Results The prepared Fe3O4@SiO2 MIPs had excellent selectivity, high adsorption capacity, and fast adsorption kinetics, reaching a maximum adsorption capacity of 4.289 mg/g at 60 min with an imprinting factor of 1.7. Fitting of the adsorption binding equation and calculation of kinetic parameters showed that the adsorption process of Fe3O4@SiO2 MIPs for DMC was in accordance with the Langmuir equation and the proposed secondary kinetic model with R2 of 0.8660 and 0.9593, respectively, and the adsorption process of Fe3O4@SiO2 MIPs for DMC belongs to chemisorption. Conclusion Fe3O4@SiO2 MIPs are a better aflatoxin enrichment material for aflatoxin detection pretreatment.