连翘叶中连翘酯苷A的提取及其抗氧化活性

研究连翘叶中连翘酯苷A的制备及其抗氧化活性。采用有机溶剂提取和柱层析方法制备粗连翘酯苷A,进一步用反相硅胶色谱柱层析得到纯化连翘酯苷A,并用高效液相色谱(high performance liquid chromatography,HPLC)测定其纯度;采用体外抗氧化活性法测定粗连翘酯苷A和纯化连翘酯苷A的1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力、羟自由基(·OH)清除能力、超氧阴离子自由基(O_2~-·)清除能力、总还原能力和抗脂质过氧化能力。从连翘叶中分离得到的纯度为62%粗连翘酯苷A和92%纯化连翘酯苷A均具有较强体外抗氧化活性,并且清除DPPH自由基、总还原能力和抗脂质过氧化能力强于阳性对照。连翘叶中连翘酯苷A含量丰富,且具有较强的抗氧化生物活性,是具有良好开发前景的天然抗氧化剂和保健食品及生物药品基料。

Extraction and Antioxidant Effect of Forsythiaside A from Forsythia suspense Leaves

The purpose of this study is to investigate the preparation of forsythiaside A and its antioxidant activity in vitro.
The crude forsythiaside A was obtained from Forsythia suspense leaves by organic solvent extraction and macroporous resin
column chromatography, and the purified forsythiaside A was obtained with reversed-phase silica column chromatography.
The crude and purified forsythiaside A were analyzed by HPLC, and their antioxidant activities in vitro were evaluated by DPPH
radical scavenging capacity, hydroxyl radical scavenging capacity, superoxide anion radical scavenging capacity, reducing power,
and anti-lipid peroxidation capacity. The 62% crude forsythiaside A and 92% purified forsythiaside A from Forsythia suspense
leaves had potent antioxidant activity, and their antioxidant activities were stronger than those of the positive control in DPPH
radical scavenging activity, reducing power, and lipid peroxidation assay. Forsythiaside A was rich in Forsythia suspense leaves,
and it had strong antioxidant activity. Therefore, forsythiaside A from Forsythia suspense leaves may be exploited as a good
natural antioxidant as well as an important functional ingredient of foods and biomedicines.