| 几种方法检测结核分支杆菌的比较 |
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| 引用本文： | 康润田,胡德华,李水仙,朱庆义.几种方法检测结核分支杆菌的比较[J].长治医学院学报,2001,15(1):52-53. |
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| 作者姓名： | 康润田 胡德华 李水仙 朱庆义 |
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| 作者单位： | 1. 长治医学院微生物学教研室,046000
2. 山西省儿童医院 |
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| 摘 要： | 目的：评价聚合酶链反应（PCR），细菌培养，抗酸染色，荧光染色对结核病的诊断价值。方法：PCR检测43例临床标本中结核分支杆菌（MTB）DNA，并与常规细菌学检测作对比。结果：PCR检测法敏感性为48.8%，荧光染色法为44.2%。细菌培养为32.6%，抗酸染色法为25.6%。结果表明，PCR法检测结核分支杆菌较抗酸染色法更敏感。结论：PCR法具有简便快速的特点。对结核病有较高的诊断价值。
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| 关 键 词： | 聚合酶链反应 结核分支杆菌 检测 |
| 修稿时间： | 2000年1月11日 |
Detection of Mycobacterium tuberculosis in Clinical Samples by Several Common Diagnostic Methods |
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| Kang Runtian,Hu Dehua,Li Shuixian et al.Detection of Mycobacterium tuberculosis in Clinical Samples by Several Common Diagnostic Methods[J].Journal of Changzhi Medical College,2001,15(1):52-53. |
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| Authors: | Kang Runtian Hu Dehua Li Shuixian |
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| Affiliation: | Kang Runtian,Hu Dehua,Li Shuixian et al. Department of medical Microbiology |
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| Abstract: | Objective:To evaluate the diagnostic value of polymerase chain reaction (PCR),culture,Semar acid fast staining,fluorescent staining in tuberculosis.Methods:Fourty three clinical Samples were detected for Mycobacterium tubeculosis and the sensitivity of PCR Technique was compared with Several common Diagnostic methods.Results: The sensitivity were 48.8% by PCR,44.2% by fluorescent staining 32.6%by culture and 25.6% by acid fast staining,respectively.PCR was found more sersitive than acid fast staining.Conclusions: PCR technique are valuable in diagnisis of tuberculisis. |
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| Keywords: | Polymerase chain reaction Mycobacterium tuberculosis sensitivity |
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