| 农杆菌介导柱型苹果“鲁加6号”遗传转化体系的建立 |
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| 引用本文： | 梁美霞,祝军,戴洪义. 农杆菌介导柱型苹果“鲁加6号”遗传转化体系的建立[J]. 分子植物育种, 2009, 7(6): 1130-1136. DOI: 10.3969/mpb.007.001130 |
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| 作者姓名： | 梁美霞 祝军 戴洪义 |
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| 作者单位： | 1. 湖南农业大学园艺园林学院,长沙,410128;青岛农业大学园林园艺学院,青岛,266109
2. 青岛农业大学园林园艺学院,青岛,266109 |
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| 基金项目： | 国家现代苹果产业技术体系项目，国家十一五科技支撑计划项目，山东省良种产业化工程项目共同资助 |
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| 摘 要： | 本研究以柱型苹果"鲁加6号"试管苗叶片外植体为转化材料,以卡那霉素抗性基凶为选择标记,建立了根癌农杆菌介导的柱型苹果"鲁加6号"苹果的遗传转化体系.研究结果表明:在MS+6-BA 4.0mg,/L+NAA 0.05 mg/L培养基上,叶片不定芽分化阶段Km的选择压力为20 mg/L,不定芽生根阶段Km的选择压力为15 mg/L,脱菌培养阶段头孢霉素的适宜浓度为300 mg/L.在农杆菌介导的遗传转化过程中,外植体经过36 h预培养,侵染10 min,共培养48 h时,有利于提高遗传转化效率,获得的抗性愈伤组织和抗性芽数均最高.获得的Km抗性植株经PCR检测,扩增出特异条带,初步证明目的基因已整合到苹果基因组DNA中.
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| 关 键 词： | 柱型苹果 根癌农杆菌 遗传转化体系 |
Establishment of Genetic Transformation System ofLujia No.6Columnar Apple Mediated by Agrobacterium tumfaciens |
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| Liang Meixia,Zhu jun,Dai Hongyi. Establishment of Genetic Transformation System ofLujia No.6Columnar Apple Mediated by Agrobacterium tumfaciens[J]. Molecular Plant Breeding, 2009, 7(6): 1130-1136. DOI: 10.3969/mpb.007.001130 |
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| Authors: | Liang Meixia Zhu jun Dai Hongyi |
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| Affiliation: | Liang Meixia 1,2 Zhu jun 2 Dai Hongyi 2 1 College of Horticulture and Landscape,Hunan Agricultural University,Changsha,410128,2 College of Landscape and Horticulture,Qingdao Agri-cultural University,Qingdao,266109 |
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| Abstract: | In this research,we established an Agrobacterium tumfaciens-mediated genetic transformation system of columnar apples named Lujia No.6,which in vitro cultured leaves were used as the explants for genetic transformation and the Kanamycin resistant gene as selective marker.The optimizing transformation system would be based on the culture media of MS+6-BA 4.0 mg/L+NAA 0.05 mg/L with the 20 mg/L Km selective pressure for differentiating the infinitive leaf shoots and 15 mg/L best for inducing their roots.The o... |
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| Keywords: | Columnar apple Agrobacterium tumfaciens Genetic transformation |
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