RhoA亚家族与胃癌细胞恶性表型的关系

目的:研究RhoA亚家族成员RhoA、RhoB和RhoC对胃癌细胞恶性表型的影响。方法:系用脂质体介导法分别将编码正显性RhoA突变体(V14RhoA)与野生型RhoB(wt-RhoB)和RhoC(wt-RhoC)的真核表达载体转染入胃癌SGC7901细胞中,筛选出稳定抗性克隆,并检测转染细胞的生物学行为的变化。结果:RhoA活性增强可促进胃癌细胞的增殖,而降低对化疗药物的敏感性;上调RhoB表达抑制胃癌细胞的增殖,增强对化疗药物的敏感性;上调表达RhoC则对胃癌细胞的增殖及对化疗药物的敏感性无明显的影响,但可明显促进胃癌细胞的迁移。结论:RhoA亚家族分子参与了胃癌生长、耐药及转移等多个方面,其家族中不同分子作用不同。

The Relationship between RhoA Subfamily Molecules and Malignant Phenotype of Gastric Cancer Cells

Objective: To explore the effect of RhoA subfamily members, i.e., RhoA, RhoB and RhoC, on the malignant phenotype of gastric cancer cells. Methods: The vectors of pCEFL-GST/V14RhoA, pCEFL-GST/wt-RhoB and pCEFL-GST/wt-RhoC were transfected into human gastric cancer cell SGC7901 by lipofectamine2000, respectively. Then the malignant biological behaviors of the transfected cells were explored. Results: The enhancement of RhoA activity can promote the proliferation of gastric cancer cell and can reduce the sensitivity of anticancer drugs; Up-regulation of RhoB can restrain the growth rate of gastric cancer cell and can enhance the sensitivity of anticancer drugs. The up-regulation of RhoC did not affect the proliferation and sensitivity of the anticancer drugs, but can promote the migration of gastric cancer cells. Conclusion: RhoA subfamily members can play an important but different role in regulating malignant behaviors of gastric cancer cells.