小鼠严重TBI后GR在大脑皮质和肝脏蛋白表达变化的差异

目的]研究小鼠闭合性严重颅脑外伤(Traumatic Brain Injury,TBI)后中枢大脑皮质糖皮质激素受体(GR)蛋白水平的变化与外周肝脏GR蛋白水平的变化、血清皮质醇(cortisol)、促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)变化的关系。方法]利用BIM-III型小型多功能动物撞击机对小鼠清醒致伤,于致伤后30min、2h、8h、24h、48h、72h采用Western Blot免疫印迹法检测大脑皮质和肝脏GR的蛋白水平的变化,酶联免疫吸附法(ELISA)检测各组外周血清中ACTH含量,放免法检测皮质醇含量。结果]致伤后大脑皮质和肝脏GR均在致伤2h后开始出现蛋白水平表达降低,8h后呈现恢复趋势,肝脏GR至72h仍未完全正常;而大脑皮质GR在致伤后24h再次呈现持续的表达降低。外周血中ACTH、皮质醇较对照组有明显升高,均具有的两个峰值特征,但是两者的高峰期并不完全吻合。结论]严重颅脑外伤后中枢和外周组织均存在糖皮质激素抵抗,两者的变化具有时相差异性。

DIFFERENT CHANGES OF GR IN CORTEX AND LIVER PROTEIN AFTER SEVERE TRAUMATIC BRAIN INJURY OF MICE

Objective]To study the differences of GR in cortex,liver protein,cortisol and ACTH after severe traumatic brain injury.Methods]Severe TBI in mice was performed by a BIM-III biomechanical machine model.At these time points:30 min,2,8,24,48 and 72h after TBI,total cytosolic GR in the cortex and liver were detected with Western blotting.Levels of seruml adrenocorticotropic hormone(ACTH)and cortisol were measured by ELISA technique and radioimmunoassay assay(RIA).Results]Both the GR in cortex and liver were ...