生长抑素受体亚型在人肝星状细胞的表达及其临床意义

目的研究人肝星状细胞系(LX-2细胞)生长抑素受体(somatostatin receptor,SSTR)亚型的表达情况,探讨其临床意义。方法选择对数生长期的LX-2细胞,将细胞以8×104个/孔接种于预置入盖玻片的6孔板中,分为LX-2组、中药复方861组、奥曲肽(octreotide)处理组及SSTR亚型阳性细胞(PANC-1)对照组,每组重复2孔。应用免疫细胞化学染色方法,观察LX-2细胞SSTR 2、SSTR5的表达;用RT-PCR方法观察SSTR亚型1~5的表达。结果免疫细胞化学染色结果发现,LX-2细胞SSTR亚型2、5表达阳性,阳性物质呈棕红色,主要位于细胞膜及细胞质,复方861处理组SSTR2、5表达增强,细胞着色较深;RT-PCR结果发现,LX-2细胞SSTR亚型1~5的表达均阳性,阳性表达的高低依次为SSTR1、SSTR4>SSTR2、SSTR5>SSTR3(P<0.05)。结论LX-2表达5种SSTR亚型,肝星状细胞可能是SST及其类似物降低门静脉压力的作用靶点。奥曲肽可能通过SSTR2、SSTR5亚型调节肝星状细胞的舒张与收缩功能,从而影响肝内阻力及门静脉压力。

Expressions of Subtypes of Somatostatin Receptors in Activated Human Hepatic Stellate Cells and Its Clinical Significance

Objective To study the expressions of somatostatin receptor subtypes in human activated hepatic stellate cells(LX-2）.Its clinical significances was also surveyed.Methods To detect the expressions of SSTR2/5 of LX-2 which is in the logarithmic growth cycle using immunocytochemistry staining method: LX-2 were routinely cultured on small glass coverslip in six well cluster dishes at a density of 8×10⁴ cells/well.The cells were divided into 5 subgroups: compound 861 0.5 g/L,compound 861 1.0 g/L,octreotide 1 mg/L and control group,PNAC-1 was a positive control cell line.Meantime,expressions of SSTR1-5 mRNA were observed using RT-PCR method.Results The expressions of SSTR 2,SSTR5 in LX-2 were positive after immunocyto-chemistry staining.The mainly staining site is cellular membrane and cellular plasm.The strong expressions in compound 861-treated group were also observed.The five kinds of subtypes of SSTR in LX-2 were proven to be positive via the detection of RT-PCR.To compared the level of the subtypes,SSTR1,4>SSTR2,5>SSTR3 was observed.Conclusion LX-2 expresses five kinds of somatostatin receptors subtype.It is suggested that the activated hepatic stellate cells is target of SST and its analogs.