Expression of tropomyosin alpha 4 chain is increased in esophageal squamous cell carcinoma as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry |
| |
| Authors: | Harada Toshio Kuramitsu Yasuhiro Makino Akira Fujimoto Masanori Iizuka Norio Hoshii Yoshinobu Takashima Motonari Tamesa Michiko Nishimura Taku Takeda Shigeru Abe Toshihiro Yoshino Shigefumi Oka Masaaki Nakamura Kazuyuki |
| |
| Affiliation: | 1. Department of Digestive Surgery and Surgical Oncology (Department of Surgery?II), Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan;2. Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan;3. Department of Bioregulatory Function, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan;4. First Department of Pathology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan |
| |
| Abstract: | To identify proteins associated with esophageal carcinogenesis, we performed protein profiling of 16 esophageal squamous cell carcinomas (ESCCs) and paired noncancerous tissues by 2-DE and MS/MS. In cancerous tissues, three spots showed significant up-regulation in the amount of protein, while eight spots were significantly down-regulated. The identities of the spots were determined by PMF with LC-MS/MS and were confirmed by immunoblotting. The up-regulated proteins were tropomyosin alpha 4 chain, transgelin, and pyruvate kinase. The down-regulated proteins were serum albumin precursor, isoforms of annexin A1, tropomyosin beta chain, 14-3-3 protein sigma, and isoforms of serotransferrin precursor. In all 16 cases, up-regulation of the tropomyosin alpha 4 chain was confirmed by immunoblotting. Localization of the tropomyosin alpha 4 chain in ESCC cells and adjacent fibroblasts was confirmed by immunohistochemistry. |
| |
| Keywords: | 2D‐PAGE Esophageal cancer LC‐MS/MS Tropomyosin |
| 本文献已被 PubMed 等数据库收录！ |
|
