Regulation and characterization of two inducible amino-acid transport systems in Chlorella vulgaris |
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Authors: | Norbert Sauer Ewald Komor Widmar Tanner |
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Affiliation: | 1. Institut für Botanik der Universit?t, Universit?tsstrasse 31, D-8400, Regensburg, Federal Republic of Germany 2. Institut für Botanik der Universit?t, Universit?tsstrasse 30, D-8580, Bayreuth, Federal Republic of Germany
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Abstract: | Glucose or non-metabolizable glucose analogues induce two amino-acid transport systems in Chlorella vulgaris: an arginine system (arginine and lysine) and a proline system (proline, glycine, alanine and serine). the same amino-acid transport systems are induced in the absence of glucose, when the cells are depleted of their nitrogen source as judged by a comparison of Km values and the lack of additive induction by the two treatments Changes in the concentration of neither internal free amino acids nor of soluble carbohydrate pools correlate prefectly with the induction of amino-acid transport. Also exogenous cAMP had no effect on the induction of transport. Both aminoacid transport systems are able to accumulate free amino acids more than 1000-fold. The accumulation plateau is not due to a steady state of influx and efflux, but rather arises by a shut-off of influx. No significant effux is observed. The biological importance of this frequently observed behaviour in amino-acid transport is discussed. |
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