款冬花多糖抗氧化能力测定

目的 用流动注射化学发光法探讨中药款冬花多糖的体外抗氧化作用。 方法 将款冬花多糖提取液加入3种化学发光体系，测量其发光强度，根据系统化学发光被抑制的程度评价款冬花对活性氧自由基的清除能力，并以抗坏血酸(Vc)为阳性对照。 结果 款冬花多糖对·OH有很好的清除作用，对O₂^-·、H₂O₂也有一定的清除作用，且在0.25~4.4 mg·L^-1内清除作用随浓度的增加而增强；对自由基的清除能力大小：·OH>H₂O₂>O₂^-·。 结论 在一定浓度范围内，款冬花多糖具有一定的抗氧化活性。

Determination of Antioxidation of Polysaccharides in Tussilago Farfara

OBJECTIVE To determine the scavenging ability on ROS free radical of polysaccharides in Tussilago farfara in vitro by flow injection chemiluminescence. METHODS The determination was based on the inhibition effect of Tussilago farfara polysaccharides extraction in three chemiluminescence system, and vitamin C was used as positive control. RESULTS It was showed that Tussilago farfara original liquid could inhibit and clear the ROS such as O₂^-·, ·OH and H₂O₂ effectively, and the efficiency was related to the concentration of polysaccharides in Tussilago farfara in the concentration range from 0.25 to 4.4 mg·L^-1. The scavenging abilities of polysaccharides in Tussilago farfara on ROS was ·OH>H₂O₂>O₂^-·. CONCLUSION The polysaccharides of Tussilago farfara have an antioxidation effect within a range of certain concentration in vitro.