SH2-B抗体对肝癌细胞脂肪沉积的影响

目的探讨SH2-B抗体对肝癌HepG2细胞内脂肪沉积的影响。方法实验分为对照组、高脂(HF)组、HF+SH2-B抗体组。将人肝癌细胞(HepG2)培养于DMEM培养基中,培养48h后用油红O染色法定性观察细胞内脂肪沉积,测定各组OD值,Western blot检测各组SH2-B蛋白表达,并用RT-PCR法检测各组JAK2mRNA表达。结果与对照组比较,HF组和HF+SH2-B抗体组肝癌细胞内脂肪含量增加(0.05),但HF组明显高于HF+SH2-B抗体组(0.05)。SH2-B蛋白和JAK2 mRNA表达在HF组明显高于HF+SH2-B抗体组和正常组(0.0 5)。结论 SH2-B蛋白及其下游分子JAK2在高脂状态下的肝细胞内表达上调,SH2-B抗体减轻肝细胞脂肪沉积可能与降低SH2-B和JAK2活性相关。

Efect of SH2-B antibody on fat accumulation in hepatic carcinoma HepG2 cells

Objective To investigate the effects of SH2-B antibody on fat accumulation in hepatic HepG2 cell. Methods HepG2 cells were cultured in DMEM medium, and divided into 3 groups： the control, high fat（HF） and HF＋anti-SH2-B groups. The fat accumulation was qualitatively analyzed in HepG2 cells using oil red O staining, Western blot was used to detect the expression of SH2-B protein and RT-PCR was used to detect the expession of JAK2 mRNA in HepG2 ceils after 48h culture. Results The fa： accumulation level was increased in HepG2 ceils in HF and HF＋anti-SH2-B groups than in control group（P〈0.05）, but was significantly increased in HF than in HF＋anti-SH2-B group （P〈0.05）. The expression levels of SH2-B protein and JAK2 mRNA in HepG2 cells were up-regnlated in HF group than in control group and HF＋anti-SH2-B groups（P〈0.05）. Conclusion The inhibition of SH2-B antibody to fat accumulation in hepatic HepG2 cells might be related to the down-regulating the expression levels of SH2-B protein and JAK2 mRNA.