树突状细胞融合在活化抗肿瘤免疫反应中的作用

目的　探讨树突状细胞 -结肠癌细胞融合在活化T细胞主要组织相容性复合体 (MHC)限制性反应中的作用。方法　使用 5 0 %聚乙二醇将人外周血树突状细胞与结肠癌细胞SW4 80融合 ,用其致敏T淋巴细胞 ,观察T细胞的活化情况。结果　融合细胞与T淋巴细胞混合后 ,CD4 Th1细胞和CD8 Tc1细胞的活化率分别为 5 7 4 5 %和 77 86 % ,而对照组无明显活化 ;T细胞的增殖明显高于对照组 (P <0 0 1) ;T细胞分泌的IFN γ明显高于对照组 (P <0 0 1)。结论　树突状 -肿瘤细胞融合策略能有效地活化抗肿瘤MHC Ⅰ类反应和Ⅱ类反应 ,促进T细胞增殖。

Dendritomas plays an important role in the activation of anti-tumor immune responses

OBJECTIVE: To investigate the role of dendritomas from fusion of human dendritic cells (DCs) and colon cancer cells in the activation of anti-tumor MHC restricted reaction. METHODS: Human dendritic cells and T cells were isolated from peripheral blood. Dendritomas were generated by fusing the human peripheral blood dendritic cells with human colon cancer cells of the SW480 line using 50% polyethylene glycol (PEG). T cells were cultured. Dendritomas and DCs were added into the culture respectively. The activation ratio of T lymphocytes was observed by MTT method. ELISA was used to detect the content of interferon-gamma (IFN-gamma) in the supernatant. RESULTS: The activation ratios of CD4(+)Th1 and CD8(+)Tc1 cells were 57.45% and 77.86 % respectively 24 hours after the T cell were mixed with dendritomas, both significantly higher than those of the controls. The proliferation of T cells mixed with dendritomas was significantly higher than that of the controls (P < 0.01); The IFN-gamma secretion in the supernatant of the T cell culture 24 hours after mixing with the dendritomas was significantly higher than that of the control (P < 0.01). CONCLUSION: The strategy of dendritomas effectively activates the anti-tumor MHC-I and MHC-II reactions and promoted the proliferation of T cells.