镇肝熄风汤对帕金森模型小鼠脑黑质 Bcl-2家族mRNA 表达的影响 |
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引用本文: | 徐天娇,;李成冲,;王晓丽,;洪博,;邹宇,;董妙先.镇肝熄风汤对帕金森模型小鼠脑黑质 Bcl-2家族mRNA 表达的影响[J].齐齐哈尔医学院学报,2014(16):2341-2342. |
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作者姓名: | 徐天娇 ;李成冲 ;王晓丽 ;洪博 ;邹宇 ;董妙先 |
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作者单位: | [1]齐齐哈尔医学院医药科学研究中心天然药物抗肿瘤研究所,161006; [2]齐齐哈尔医学院卫生所,161006; |
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基金项目: | 国家自然科学基金资助项目(No.81241107,No.81373629) |
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摘 要: | 目的:观察镇肝熄风汤对1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methy-4-phenyl-1,2,3,6-tetrahy-dropyridine ,MPTP)诱导的帕金森病( PD)模型小鼠脑黑质Bcl-2家族蛋白表达的影响。方法采用连续灌胃给予C57BL/6小鼠不同剂量镇肝熄风汤(12.5、25和50 g/kg)14天,于第11天开始,给药前1 h皮下注射MPTP(30 mg/kg),连续4天,造模后运用实时定量PCR法检测Bax、Bad、Bcl-2和Bcl-xl mRNA的表达。结果 PCR结果显示,镇肝熄风汤下调了Bax mRNA表达(P<0.05),同时上调了Bcl-2和Bcl-xl mRNA表达(P<0.05),对Bad mRNA表达无影响。结论镇肝熄风汤具有抑制PD模型小鼠脑黑质Bax mRNA表达,促进Bcl-2和Bcl-xL mRNA表达的作用。
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关 键 词: | 帕金森病 镇肝熄风汤 Bcl-2家族 |
Effect of Zhenganxifeng decoction on expression of Bcl-2 family mRNA in substantia nigra of MPTP-induced mice |
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Affiliation: | XU Tian-jiao, et al. (Medical Science Research Center, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China.) |
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Abstract: | Objective To investigate the effects of Zhenganxifeng decoction on expression of Bcl -2 family mRNA in the substantia nigra of MPTP-induced C57BL/6 mice.Methods C57BL/6 mice were given MPTP (30 mg/kg) to prepare Parkinson disease mice model .Different doses of Zhenganxifeng decoction (12.5, 25, and 50 g/kg) were given 14 d prior to MPTP in the pretreatment groups .Real-time PCR was used to analyze the expression of Bax , Bad, Bcl-2 and Bcl-xl mRNA.Results Zhenganxifeng decoction could down-regulate the expression of Bax mRNA , and up-regulate the expression of Bcl-2 and Bcl-xL mRNA.Conclusions The results suggest that Zhenganxifeng decoction could suppress the expression of Bax mRNA and Promote the expression of Bcl-2 and Bcl-xl mRNA in substantia nigra of Parkinson&'s disease mice induced by MPTP . |
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Keywords: | Parkinson disease Zhenganxifeng decoction Bcl-2 family |
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