尿道致病性大肠杆菌和奇异变形杆菌对人尿道上皮细胞的粘附

作者测定和比较了具有P菌毛的尿道致病性大肠杆菌,具有Ⅰ型菌毛的普通大肠杆菌和奇异变形杆菌在体外粘附正常人尿道上皮细胞的能力,对测定细菌粘附能力的方法和影响细菌粘附的因素进行了讨论。

ADHERENCE OF UROPATHOGENIC ESCHERICHIA COLI AND PROTEUS MIRABILIS TO HUMAN UROEPITHELIAL CELLS

Adherence of Escherichia coli and Pro-teus mirabilis to human uroepithelial cells invitro was studied. Uroepithelial cells fromfresh morning urine of healthy young girlsand from mucous membrane of ureter of thedonors were used. The P. mirabilis strainsadhered only to squamous cells and not totransitional epithelial cells. P-fimbriated uro-pathogenic E. coli strains adhered to boththe cell types in a great quantity, whereastype-I fimbriated common E. coli strainsadhere? to either of the cell types verypoorly, but were heavily trapped by normalurinal slime. Difference in adhesion charac-teristics between the fimbriated E.coli andthe P. mirabilis may be related to the dif-ferent clinical manifestation of the urinarytract infection. Optimal test conditions wereobthained with post-log-phase bacterial cul-tures grown on the nutrient broth and nowashing of the bacteria before incubationwith the epithelial cells. The measure of theadherence of bacteria to the surface of theepithelial cells with the stained smear wasmore convenient and accurate than with thewet film,and the problem whether thecounts under microscope are those of thebacteria attached to the surface of theepithetial cells or of the inclusions in theepithelial cells could be solved.