| 苜蓿花叶病毒复制酶基因全长cDNA植物表达载体的构建和对烟草的转化 |
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| 引用本文： | 张竞秋,邢燕平,哈斯阿古拉. 苜蓿花叶病毒复制酶基因全长cDNA植物表达载体的构建和对烟草的转化[J]. 内蒙古大学学报(自然科学版), 2004, 35(4): 423-427 |
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| 作者姓名： | 张竞秋 邢燕平 哈斯阿古拉 |
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| 作者单位： | 内蒙古大学生命科学学院,呼和浩特,010021;内蒙古农业大学生物工程学院,呼和浩特,010019 |
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| 基金项目： | 国家自然科学基金资助项目(39860042) |
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| 摘 要： | 将苜蓿花叶病毒中国分离株(Alfalfa mosaic virus Chinese isolate，A1MV-Ch)的复制酶P2亚基(90 kD蛋白)基因的全长cDNA构建到植物表达载体pROKⅡ中，得到重组植物表达载体pAIMV-FL．用三亲融合法导入农杆菌LBA4404，并转化烟草，经PCR检测，获得了含全长cDNA的转基因烟草植抹．
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| 关 键 词： | 苜蓿花叶病毒 复制酶基因 植物表达载体 转基因 |
| 文章编号： | 1000-1638(2004)04-0423-05 |
| 修稿时间： | 2003-09-08 |
Construction of Plant Expression Vector of the Full Length cDNA of AlMV Replicase Gene and the Transformation of Tobacco |
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| ZHANG Jing-qiu,XING Yan-ping,Hasi Agula. Construction of Plant Expression Vector of the Full Length cDNA of AlMV Replicase Gene and the Transformation of Tobacco[J]. Acta Scientiarum Naturalium Universitatis Neimongol, 2004, 35(4): 423-427 |
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| Authors: | ZHANG Jing-qiu XING Yan-ping Hasi Agula |
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| Affiliation: | ZHANG Jing-qiu~1,XING Yan-ping~2,Hasi Agula~1 |
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| Abstract: | The full length cDNA of the replicase gene which encodes 90kD proteins in the AlMV was recombinated with the plant expression vector pROKII to obtain the recombinated vector pAlMV-FL.Then the ALMV replicase gene was introduced into the tobacco cultivar via Agrobacterium tumefaciens by Triparental mating method.The PCR amplification of transgenic tobacco plants showed that the full length cDNA of replicase gene was integrated into the genome of transgenic tobacco plants. |
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| Keywords: | Alfalfa mosaic virus replicase gene plant expression vector transgenic |
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