High performance liquid chromatography fingerprints of polysaccharide hy⁃ drolysate monosaccharides in Mesona chinensis BenthEI北大核心CSCD

：Based on the pre-column derivatization of monosaccharides with 1-phenyl-3-methyl-5-pyrazolone，the fingerprints of polysaccharide hydrolysate monosaccharides in the extract of Mesona chinensis Benth was established using reversed-phase high performance liquid chromatography （HPLC） gradient elution/UV detection technology，which was used for the analysis of the components and content of polysaccharide hydrolyzed monosaccharides in the aqueous extract of Mesona chinensis Benth，and eight monosaccharide components including ribose，rhamnose，glucuronic acid，galacturonic acid，glucose，galactose，xylose and arabinose were identified. Similarity analysis，cluster analysis，principal component analysis of the HPLC fingerprints of monosaccharide components were carried out，and the changes in the contents of galactose， galacturonic acid and glucose monosaccharide components in various samples were clarified. Regional differences lead to differences in cluster analysis，and there are certain differences in the content and distribution of monosaccharide components produced by polysaccharide hydrolysis in Mesona chinensis Benth collected from different origins. The method can provide a potential technology for characterizing monosaccharide components of the polysaccharide in aqueous extract of Mesona chinensis Benth. © 2023, Youke Publishing Co.,Ltd. All rights reserved.