| 乙型肝炎病毒前C区变异对HepG2细胞HLA-I表达的影响 |
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| 引用本文： | 林裕龙,骆抗先,侯金林,陈伟红.乙型肝炎病毒前C区变异对HepG2细胞HLA-I表达的影响[J].中华肝脏病杂志,2002(5). |
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| 作者姓名： | 林裕龙 骆抗先 侯金林 陈伟红 |
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| 作者单位： | 第一军医大学南方医院传染科
510515广州(林裕龙，骆抗先，侯金林)，第四军医大学唐都医院传染科 710032西安(陈伟红) |
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| 摘 要： | 目的 研究乙型肝炎病毒（HBV）前 C区热点变异对宿主细胞HLA—I分子表达的影响。方法 构建HBV前C区野毒株及A83、A83/A86变异株真核表达质粒,转染HePG2细胞,鉴定目的基因在宿主细胞中的生物活性,流式细胞术检测HLA—I分子的表达。结果 PCR和 ELISA法分析能检测到目的DNA片段和HBeAg,转染细胞表达HLA—I分子的平均荧光强度不同,野毒株为1.3,前C区变异后平均荧光强度增加,尤以A83变异表达增强显著（17.6）,A83/A86为7.3。结论 前C区热点变异后宿主细胞HLA—I分子的表达为上调。
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| 关 键 词： | 肝炎病毒 乙型 基因 变异 HLA-I分子 |
Influence of mutation in HBV precore region on the expression of HLA- I in HepG2 cells |
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| CHEN Weihong,LINYulong,LUO Kangxian,HOU Jinlin.Influence of mutation in HBV precore region on the expression of HLA- I in HepG2 cells[J].Chinese Journal of Hepatology,2002(5). |
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| Authors: | CHEN Weihong LINYulong LUO Kangxian HOU Jinlin |
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| Affiliation: | CHEN Weihong,LINYulong,LUO Kangxian,HOU Jinlin. Department of Infectious Diseases,Nanfang Hospital,First Military Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To study the influence of host cellular HLA- I molecules expression by HBV precore region mutants. Methods The plasmids of the wild type of HBV precore region and the mutants of A83 and A83/A86 were constructed, and then transected into HepG2 cells. The biological activity of HBV precore gene in the host cells was identified. The expression of HLA- I molecules was detected by flow cytometric analysis. Results DNA segments similar with HBV precore gene size and HBeAg were detected by PCR and ELISA, respectively. The fluorescence intensity of HLA- I on host cells was different: wild type being 1.3; A83, 17.6; and A83/A86, 7.3. Conclusions HBV precore gene hot-spot mutants in vitro can increase the expression of HLA-I molecules on host cells. |
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| Keywords: | Hepatitis B virus Genes Mutation HLA- I molecules |
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