| 菊花品种神马的茎段离体快繁研究 |
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| 引用本文： | 张天静,雷家军,孙文松. 菊花品种神马的茎段离体快繁研究[J]. 黑龙江农业科学, 2014, 0(12): 19-22 |
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| 作者姓名： | 张天静 雷家军 孙文松 |
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| 作者单位： | 1. 辽宁省经济作物研究所,辽宁辽阳,111000
2. 沈阳农业大学园艺学院,辽宁沈阳,110866 |
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| 基金项目： | 沈阳市农业科技共建资助项目(201423) |
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| 摘 要： | 为加快优质菊花种苗的繁殖,以菊花品种神马的茎段为试材,建立了无菌操作体系,并进行继代增殖、生根培养和驯化移栽。结果表明:升汞消毒茎段5min效果最佳,存活率可达70.0%,污染率为13.30%;最佳初代培养基为MS+BA 1.0mg·L-1+NAA 0.1mg·L-1,萌发率高且生长良好;最佳增殖培养基为MS+BA1.0mg·L-1+NAA0.1mg·L-1,增殖系数达到6.2;最佳生根培养基为1/2MS+NAA0.1mg·L-1,生根率可达97.8%。
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| 关 键 词： | 菊花 茎段 离体快繁 |
Study on Stem Micropropogation of Chrysanthemum cv.Jinba |
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| ZHANG Tian-jing , LEI Jia-jun , SUN Wen-song. Study on Stem Micropropogation of Chrysanthemum cv.Jinba[J]. Heilongjiang Agricultural Science, 2014, 0(12): 19-22 |
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| Authors: | ZHANG Tian-jing LEI Jia-jun SUN Wen-song |
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| Affiliation: | ZHANG Tian-jing , LEI Jia-jun ,SUN Wen-song (1. Economic Crop Institution of Liaoning Province, Liaoyang, Liaoning 111000;2. College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866) |
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| Abstract: | In order to speed up the breeding of high-quality Chrysanthemumcv.Jinba,taking stem segment of Chrysanthemumcultivar Jinba materials,sterile system was established,and the axillary bud in vitro was subcultured,rooted,domesticated and transplanted.The results showed that the disinfection time by 0.1% HgCl2 for 5minutes was optimal,and the survival rate could be up to 70.0%,and contamination rate was 13.3%.When the induction medium was MS＋BA 1.0mg·L-1＋NAA 0.1mg·L-1,the germination rate was high and the plantlets grew well.The optimal subculture medium was MS＋BA1.0mg·L-1＋NAA0.1mg·L-1 with multiplication coefficient of 6.2,and the most suitable rooting medium was 1/2MS＋NAA0.1mg·L-1,with rooting rate over 97.8%. |
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| Keywords: | Chrysanthemumcv.Jinba stem rapid propogation in vitro |
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