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长三角地区粳稻种质的稻瘟病抗性基因鉴定及其抗性评价
引用本文:闫影,王凯,周锋利,张丽霞,胡泽军,曹黎明,吴书俊. 长三角地区粳稻种质的稻瘟病抗性基因鉴定及其抗性评价[J]. 核农学报, 2022, 36(1): 14-23. DOI: 10.11869/j.issn.100-8551.2022.01.0014
作者姓名:闫影  王凯  周锋利  张丽霞  胡泽军  曹黎明  吴书俊
作者单位:1上海市农业科学院作物育种栽培研究所,上海 2014032上海农产品保鲜加工工程技术研究中心,上海 2014033上海市嘉定区农业技术推广服务中心,上海 201800
基金项目:上海市科技兴农重点攻关项目[沪农科攻字(2016)第6-1-1号];
摘    要:已克隆的抗稻瘟病基因Pi9、Pib、Pi5、Pita、Pi2、Pikh、Pikm和Pigm对稻瘟病菌表现广谱抗性,这些基因已广泛应用于水稻抗稻瘟病育种.为了明确长三角地区粳稻的抗稻瘟病基因分布及其抗性表现,利用上述8个抗病基因的功能标记对52份粳稻种质进行抗病基因检测.同时,利用江苏省2019年稻瘟病菌代表菌株2019...

关 键 词:粳稻  稻瘟病  抗性基因  稻瘟病菌  抗性评价
收稿时间:2020-10-16

Identification and Resistance Evaluation of Rice Blast Resistance Genes in Japonica Rice Germplasm in Yangtze River Delta
YAN Ying,WANG Kai,ZHOU Fengli,ZHANG Lixia,HU Zejun,CAO Liming,WU Shujun. Identification and Resistance Evaluation of Rice Blast Resistance Genes in Japonica Rice Germplasm in Yangtze River Delta[J]. Acta Agriculturae Nucleatae Sinica, 2022, 36(1): 14-23. DOI: 10.11869/j.issn.100-8551.2022.01.0014
Authors:YAN Ying  WANG Kai  ZHOU Fengli  ZHANG Lixia  HU Zejun  CAO Liming  WU Shujun
Affiliation:1Institute of Crop Breeding and Cultivation, Shanghai Academy of Agricultural Sciences, Shanghai 2014032Shanghai Agricultural Products Preservation Prcessing Engineering Technology Research Center, Shanghai 2014033Jiading District Agro-Technology Extension Service Center, Shanghai 201800
Abstract:The cloned rice blast resistance genes,Pi9、Pib、Pi5、Pita、Pi2、Pikh、Pikm and Pigm,which showed broad-spectrum blast resistance in different rice growing regions,and had been widely used in rice breeding for blast resistance.In order to determine the distribution and resistance performance of rice blast resistance genes in japonica rice in the Yangtze River Delta,52 japonica rice germplasms were tested by using the functional markers of the above 8 disease resistance genes.At the same time,the spore mixtures of representative strains of rice blast,2019-746,2019-587,2019-924,2019-16-3,2019-522 and 2019-863 in Jiangsu province in 2019,were used to identify the resistance of the tested germplasms.The results showed that Pib and Pita,which had higher distribution frequencies of 78.85%and 50.00%respectively in the tested germplasms,and the distribution frequencies of other genes were 38.46%(Pikh),34.62%(Pi9),23.08%(Pikm),21.15%(Pi2)and 5.77%(Pi5),respectively.Pigm gene was not carried in all the tested germplasms.The results suggested that the tested germplasms with disease-resistant genes Pita,Pi5,Pi2 and Pikm showed a high proportion of disease resistance.Pita,Pi5,Pi2 and Pikm can be used as the main genes to improve rice blast resistance in future.The results of this study provide a scientific basis for the utilization of rice blast resistance genes in the Yangtze River Delta.
Keywords:japonica rice  rice blast  resistance gene  Magnaporthe oryzae  resistant evaluation
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