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正交试验法优选从豆制品下脚料中提取大豆皂苷的工艺
引用本文:陈燕军,张毅贞,卢汝梅,戴航,李健,迟嘉松.正交试验法优选从豆制品下脚料中提取大豆皂苷的工艺[J].中草药,2001,32(7):602-604.
作者姓名:陈燕军  张毅贞  卢汝梅  戴航  李健  迟嘉松
作者单位:广西中医学院,
基金项目:广西自治区卫生厅1999年青年基金课题
摘    要:目的 优选从豆制品下脚料中提取大豆皂苷的最佳提取工艺条件。方法 通过正交试验,以紫外分光光度法测定总皂苷含量为指标,对提取工艺进行考察。结果 影响回流提取的主要因素对乙醇浓度;影响渗漉提取的因素依次为乙醇浓度、溶媒量和渗漉速度。结论 最佳提取工艺条件为8倍量60%乙醇,热回流1次2.5h。

关 键 词:豆制品下脚料  大豆皂苷  提取工艺  正交设计  紫外分光光度法
文章编号:0253-2670(2001)07-0602-03
收稿时间:2000/9/14 0:00:00
修稿时间:2000年9月14日

Opt imization of processes for recovery of soyasaponins from bean product waste by orthogonal design
CHEN Yan-jun,ZHANG Yi-zhen,LU Ru-mei,DAI Hang,LI Jian and CHI Jia-song.Opt imization of processes for recovery of soyasaponins from bean product waste by orthogonal design[J].Chinese Traditional and Herbal Drugs,2001,32(7):602-604.
Authors:CHEN Yan-jun  ZHANG Yi-zhen  LU Ru-mei  DAI Hang  LI Jian and CHI Jia-song
Affiliation:Guangxi University of TCM, Nanning Guangxi 530001, China;Guangxi University of TCM, Nanning Guangxi 530001, China;Guangxi University of TCM, Nanning Guangxi 530001, China;Guangxi University of TCM, Nanning Guangxi 530001, China;Guangxi University of TCM, Nanning Guangxi 530001, China;Guangxi University of TCM, Nanning Guangxi 530001, China
Abstract:Object To optimize the process for the recovery of soyasaponins from bean product waste. Methods Conditions for the recovery were studied by orthogonal design guided by the yield of soya-saponins as determined by UV absorbance. Results The most influential factor in the recovery by refluxing was the concentration of alcohol; while in the recovery by percolation the influential factors were in the order of concentration of ethyl alcohol, amount of alcohol used and the rate of percolation.Conclusion The optimal recovery process was found to be a single reflux with eight times of 60% alcohol for 2.5 h.
Keywords:bean product waste  soyasaponins  extraction process  orthogonal design  ultraviolet spectrophotometry  
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