男科门诊患者人乳头瘤病毒感染基因型分析

目的 研究男科门诊患者人乳头瘤病毒（humanpapillomavirus,HPV）感染及其基因型的分布状况，同时与女性感染状况进行比较分析，为临床制定男性HPV相关性疾病的防治措施提供科学依据。方法 采用聚合酶链反应（polymerase chain reaction,PCR）扩增后导流杂交的方法对2018年7月至2022年12月就诊于扬州市妇幼保健院男科门诊的258例男性患者进行37种HPV基因分型检测，检测结果与同期送检的1436例女性体检者及931例宫颈疑似HPV感染的妇科患者宫颈脱落细胞样本检测结果进行比较。结果 258例男科门诊患者中HPV阳性者103例，感染率为39.92%。103例阳性样本中感染HPV高危型、低危型和高低危混合型各占58.25%、20.39%、21.36%，其中单一亚型感染59例，占57.28%，二重及以上感染（多重感染）44例，占42.72%，最多十重感染。单一亚型感染以高危型为主，二重及以上感染（多重感染）以高危型及高低危混合型为主。男性患者HPV感染率和感染类型及感染状态与女性患者相比差异无统计学意义（P=0.456,0.192,0.102）；与女...

Genotyping analysis of human papillomavirus infection in male outpatient patients

Objective To study the human papillomavirus (HPV) infection and genotype distribution characteristics in male outpatients, and compare with female infection status, in order to provide scientific basis for the clinical development of prevention and treatment measures for male HPV infection related diseases. Methods Polymerase chain reaction (PCR) amplification followed by directed hybridization was used to detect 37 HPV genotypes in 258 male outpatients of Yangzhou Maternal and Child Health Hospital from July 2018 to December 2022.The detection results were further compared with the detection results of 1436 female physical examinees and 931 cervical exfoliated cell samples of gynecological patients suspected of HPV infection at the same time. Results There were 103 of the 258 male outpatients were positive, with an infection rate of 39.92%. Among the 103 positive samples, high-risk, low-risk, and mixed high-risk HPV infections accounted for 58.25%, 20.39%, and 21.36%, respectively. Among them, 59 were infected with single infection, accounting for 57.28%, 44 were infected with more than double infection (multiple infection), accounting for 42.72%, and the most one had ten types infections. Single infection was mainly high-risk type, while multiple infection was mainly high-risk type and mixed high-risk type. There was no statistically significant difference in HPV infection rate, infection type, and infection status between male patients and female patients (P=0.456, 0.192, 0.102), but there was a statistically significant difference compared with female physical examinees (P <0.001, 0.032, <0.001). The peak age of HPV infection is 20-39 years old, accounting for 74.75%, and there was no statistically significant difference in HPV detection rate among different age groups (P=0.297). 33 HPV genotypes were detected in both male and female groups. The top five subtypes were HPV52,58,51, 54 and 61 in male patients, while HPV57, 67, 69 and 83 were not detected. The top five subtypes were HPV52, 16, 58, 53 and 61 in female groups, while HPV57, 69, 72 and 26 were not detected. Physical examination is the main reason of 103 male infected patients seeking medical treatment. Conclusion The HPV infection status in male is similar to that of female patients, with high-risk infection being the main type and single subtype infection being the main infection. The peak age of male patients with HPV infection is 20-39 years old. HPV52, 58, 51, 54, 61 are the most common types, and most of them are asymptomatic. Therefore, men are high-risk groups of HPV infection. It is necessary to carry out HPV detection for male outpatients.