7-氨基头孢烷酸中蛋白质含量的检测

7-氨基头孢烷酸(7-ACA)为生产半合成头孢类抗生素的重要母核,其质量情况直接决定了各类合成头孢产品生产质量水平。为监控产品质量,采用考马斯亮蓝(Coomassie Brilliant Blue)法对7-氨基头孢烷酸(7-ACA)中蛋白质浓度进行检测,并对检测方法进行验证:检测波长为595 nm,蛋白质含量为0.000～100.000μg呈良好线性,相关系数r=0.999 3,平均回收率为99.3%(n=3)。采用考马斯亮蓝法对7-氨基头孢烷酸(7-ACA)中蛋白质浓度进行检测,灵敏度高,准确、可靠,可作为7-氨基头孢烷酸的质量控制方法。

Determination on Content of Protein in 7-ACA

7-ACA is an important mother nucleus of semi-synthetic cephalosporin antibiotics,the quality of which directly determines the quality of various synthetic cephalosporin products.In order to monitor the quality of products,Coomassie Brillian Blue method was used to determine the concentration of protein in 7-ACA,and the detection method was verified: when detect wavelength was 595 nm,the linearity range was 0.000～100.000 μg,the correlation was 0.9993,and the average recovery rate was 99.3%(n=3).This method had the advantages of high precision and accuracy,and it was suitable for the analysis of the concentration of protein in 7-ACA.As a result,the Coomassie Brillian Blue method could be the method to control the quality of 7-ACA.