| CLONING AND SEQUENCING OF IMMUNOGLOBULINVARIABLE－REGION GENE OF A MONOCLONALANTIBODY SPECIFIC FOR HUMANHEPATOCARCINOMA |
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| 引用本文： | 杨萍,高磊,胡川闽,刘彦仿,陈苏民,陈南春. CLONING AND SEQUENCING OF IMMUNOGLOBULINVARIABLE－REGION GENE OF A MONOCLONALANTIBODY SPECIFIC FOR HUMANHEPATOCARCINOMA[J]. 中国癌症研究, 1996, 8(1): 1-4. DOI: 10.1007/BF02674956 |
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| 作者姓名： | 杨萍 高磊 胡川闽 刘彦仿 陈苏民 陈南春 |
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| 作者单位： | Yang Ping;Gao Lei; Hu Chuanmin; Liu Yanfang;Chen Sumin; Chen NanchunDepartment of pathology，Department of molecular Biology，The Fourth Military Medical University，Xi’an 710032 |
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| 摘 要： | ＣＬＯＮＩＮＧＡＮＤＳＥＱＵＥＮＣＩＮＧＯＦＩＭＭＵＮＯＧＬＯＢＵＬＩＮＶＡＲＩＡＢＬＥ－ＲＥＧＩＯＮＧＥＮＥＯＦＡＭＯＮＯＣＬＯＮＡＬＡＮＴＩＢＯＤＹＳＰＥＣＩＦＩＣＦＯＲＨＵＭＡＮＨＥＰＡＴＯＣＡＲＣＩＮＯＭＡＹａｎｇＰｉｎｇ杨萍；ＧａｏＬｅｉ高...
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| 修稿时间： | 1995-07-20 |
Cloning and sequencing of immunoglobulin variable-region gene of a monoclonal antibody specific for human hepatocarcinoma |
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| Yang Ping,Gao Lei,Hu Chuanmin,Liu Yanfang,Chen Sumin,Chen Nanchun. Cloning and sequencing of immunoglobulin variable-region gene of a monoclonal antibody specific for human hepatocarcinoma[J]. Chinese Journal of Cancer Research, 1996, 8(1): 1-4. DOI: 10.1007/BF02674956 |
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| Authors: | Yang Ping Gao Lei Hu Chuanmin Liu Yanfang Chen Sumin Chen Nanchun |
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| Affiliation: | (1) Department of Pathology, The Fourth Military Medical University, 710032 Xi'an;(2) Department of molecular Biology, The Fourth Military Medical University, 710032 Xi'an |
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| Abstract: | A murine monoclonal antibody HAb27 specific for human hepatocarcinoma has been developed for radio-immunolocalization in animal models. The isotype of this antibody was IgG1, κ. In the present study, we used a set of oligonucleotide primers to amplify the cDNA of mouse immunoglobulin heavy and light chain variable region genes by the polymerase chain reaction. Sequence analysis of the heavy variable region indicated that the VH region was highly homologous to the plasmacytoma cell line MOPC21 gene, and closely related to germline genes of the VHIII family. The JH region was encoded by the JH3 gene. For the light chain, the Vκ segment of the antibody showed the highest homology to the germline VκOx1 gene, and the Jκ region was Jκ5. |
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| Keywords: | Monoclonal antibody Immunoglobulin variable region gene PCR Hepatocarcinoma |
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