| 人乙肝病毒前表面抗原PreS1在家蚕培养细胞中的表达 |
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| 引用本文： | 夏学春,陈健,张耀洲. 人乙肝病毒前表面抗原PreS1在家蚕培养细胞中的表达[J]. 蚕业科学, 2005, 31(1): 103-106 |
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| 作者姓名： | 夏学春 陈健 张耀洲 |
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| 作者单位： | 浙江理工大学生物化学研究所,杭州,310018 |
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| 摘 要： | 人乙肝病毒前表面抗原PreS1诱导的免疫反应可以克服机体对乙肝病毒S蛋白的无反应状态。将人乙肝病毒adr前表面抗原preS1基因克隆到杆状病毒转移载体pBacPAK8中,获得重组转移载体pBacPAKpreS1。用此转移载体与线性化病毒BmBacPAK6线性化基因组DNA共转染单层家蚕细胞(BmN),经细胞内同源重组和空白筛选,获得重组病毒。ELISA结果表明重组蛋白PreS1在家蚕培养细胞中的表达水平为02pg/个细胞,Western印迹证实此蛋白大小约为14kD。
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| 关 键 词： | 人乙肝病毒 前表面抗原PreS1 家蚕培养细胞 表达 |
| 文章编号： | 0257-4799(2005)01-0103-04 |
| 修稿时间： | 2004-06-08 |
Expression and Identification of Human Hepatitis B Virus Protein PreS1 in Silkworm Bombyx mori Cell |
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| XIA Xuechun,CHEN Jian,ZHANG Yaozhou. Expression and Identification of Human Hepatitis B Virus Protein PreS1 in Silkworm Bombyx mori Cell[J]. Acta Sericologica Sinica, 2005, 31(1): 103-106 |
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| Authors: | XIA Xuechun CHEN Jian ZHANG Yaozhou |
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| Abstract: | The immunoresponsiveness induced by PreS1 of HbsAg can bypass nonrespone to the S region of HbsAg.preS1 gene was cloned into baculovirus transfer vector pBacPAK8,resulting in a recombinant transfer vector pBacPAKpreS1.The vector pBacPAKpreS1 and linearized DNA of modified baculovirus Bm-BacPAK were cotransfected into the cultured Bombyx mori(Bm)N cells in which homologous recombination occurred.Then the recombinant baculovirus were screened out.The BmN cells were infected with the baculovirus recombinant VpreS1.The results of ELISA and Western immunoblotting assays revealed that the expression levels of the recombinant protein PreS1 in BmN cells could be 0.2 pg/cells,which presented at 14 kD. |
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| Keywords: | Hepatitis B virus PreS1 Cultured Bomyx mori cell Expression |
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