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| 自然发酵蔬菜制品中降解亚硝酸盐乳酸菌的分离与鉴定 | |
| 引用本文： | 朱军莉,赵 进,朱雅霏.自然发酵蔬菜制品中降解亚硝酸盐乳酸菌的分离与鉴定[J].中国酿造,2015,34(6):39. |
| 作者姓名： | 朱军莉 赵 进 朱雅霏 |
| 作者单位： | 1.浙江工商大学食品与生物工程学院，浙江杭州310018；2.中国计量学院生命科学学院，浙江杭州310018； 3.浙江蔡小珍农业开发有限公司，浙江建德311600 |
| 基金项目： | 杭州市科技局项目（30130432B104） |
| 摘 要： | 以自然发酵蔬菜为原料，采用平板计数和钙溶圈法分离纯化菌株，并分析菌株的亚硝酸盐降解能力。结果表明，钙溶圈法分离的产酸能力较强的9株细菌，经生理生化鉴定均为乳酸菌。乳酸菌在MRS培养基中都具有亚硝酸盐分解能力，其中H12、L7和X1三株乳酸菌亚硝酸盐降解率高于89%，且降解速度较快，其中X1降解亚硝酸活性最强。乳酸菌X1还表现良好的产酸能力和耐盐性，经16S rDNA鉴定为植物乳杆菌(Lactobcillus plantarum)。 |
| 关 键 词： | 乳酸菌 亚硝酸盐降解 分离 鉴定 |
Isolation and identification of nitrite-degrading lactic acid bacteria from natural fermented vegetable |
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| ZHU Junli,ZHAO Jin,ZHU Yafei.Isolation and identification of nitrite-degrading lactic acid bacteria from natural fermented vegetable[J].China Brewing,2015,34(6):39. | |
| Authors: | ZHU Junli ZHAO Jin ZHU Yafei |
| Affiliation: | 1.College of Food Science and Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310038, China; 2.College of Life Science, China Jiliang University, Hangzhou 310018, China; 3.Zhejiang Caixiaozhen Agricultural Development Company, Jiande 311600, China |
| Abstract: | Bacteria with high nitrite degradation ability were isolated from natural fermented vegetable by plate count method and calcium dissolved ring method. Nine strains with strong acid production ability were screened and identified as lactic acid bacteria by physiological and biochemical identification. The nitrite degradation ability in MRS liquid medium differed among nine strains, strain H12, L7 and X1 exhibited rapid nitrite degradation rate of higher than 89%. Strain X1 showed the strongest ability for nitrite degradation, and it also showed strong ability of acid production and salt resistance, which was identified as Lactobcillus plantarum by 16S rDNA sequencing. |
| Keywords: | lactic acid bacteria nitrite degeneration isolation identification |
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