| MAP-H2O2-HRP伏安酶联免疫分析测定南方菜豆花叶病毒 |
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| 引用本文： | 张书圣,焦奎,陈洪渊,王曼霞,张成良,张作芳.MAP-H2O2-HRP伏安酶联免疫分析测定南方菜豆花叶病毒[J].高等学校化学学报,2000,21(8):1200-1204. |
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| 作者姓名： | 张书圣 焦奎 陈洪渊 王曼霞 张成良 张作芳 |
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| 作者单位： | 1. 青岛化工学院应用化学系, 青岛 266042;
2. 南京大学化学系, 南京 210093;
3. 农业部植物检疫实验所, 北京 100029 |
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| 基金项目： | 国家自然科学基金，山东省青年科研项目，29775012，Q99B16，， |
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| 摘 要： | 提出间氨基酚(MAP)-H2O2-辣根过氧化物酶(HRP)伏安酶联免疫分析新体系,并用于南方菜豆花叶病毒(SBMV)的测定.以线性扫描二阶导数伏安法检测HRP催化H2O2氧化MAP的产物,用于游离HRP及SBMV的测定,灵敏度均高于经典的ELISA显色光度法.本法对HRP测定的线性范围为1.0×10-8～1.0×10-6g/L,检测限为3.8×10-9g/L;对SBMV测定的线性范围为4.0～5000ng/mL,检测限为4.0ng/mL.用所建立的方法测定病毒感染病叶澄清液的最高稀释比为1∶1.5×105,并与现行的ELISA显色光度法进行对照,二者相关性很好.
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| 关 键 词： | 南方菜豆花叶病毒 辣根过氧化物酶 间氨基酚 免疫分析 伏安法 |
| 文章编号： | 0251-0790(2000)08-1200-05 |
| 收稿时间： | 1999-11-17 |
MAP-H2O2-HRP Voltammetric Enzyme-linked Immunoassay for Trace Southern Bean Mosaic Virus |
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| ZHANG Shu-Sheng,JIAO Kui,CHEN Hong-yuan,WANG Man-Xia,ZHANG Cheng-liang,ZHANG Zuo-Fang.MAP-H2O2-HRP Voltammetric Enzyme-linked Immunoassay for Trace Southern Bean Mosaic Virus[J].Chemical Research In Chinese Universities,2000,21(8):1200-1204. |
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| Authors: | ZHANG Shu-Sheng JIAO Kui CHEN Hong-yuan WANG Man-Xia ZHANG Cheng-liang ZHANG Zuo-Fang |
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| Affiliation: | 1. Departmentof App lied Chemistry, Qingdao Instituteof Chemical Technology, Qingdao 266042, China;
2. Departmentof Chemistry, Nanjing University, Nanjing 210093, China;
3. Instituteof Plant Quarantine, Ministry of Agriculture, Beijing 100029, China |
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| Abstract: | Avoltammetric enzyme-linked immunoassay based on the new system of m-aminophenol(MAP)-H2O2-horseradish peroxidase(HRP) has been developed and used for the detection of HRPand southern bean mosaic virus(SBMV). HRPor labelled HRPcatalyzes the oxidation reaction of MAPwith H2O2, the product of which produces a sensitive voltammetric peak at potential of -0.46V(vs. SCE ) in Britton-Robinson(B-R) buffer solution.By using this voltammetric peak, HRPcan be measured with a detection limit of 38×10-9 g/Land a linear range of 10×10-8 -10×10-6 g/L. The detection limit for the clarified SBMVis 4.0 ng/mLand the linear range of 4.0-5000 ng/mL. The highest dilution ratio detected for the infected leaf sap is 1∶1.5×105. |
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| Keywords: | Southern bean mosaic virus(SBMV) Horseradish peroxidase(HRP) m Aminophenol Immunoassay Voltammetry |
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