白细胞介素(IL)-2与IL-4对小鼠膀胱癌肿瘤浸润性淋巴细胞增殖及细胞毒性的协同调节作用

目的 探讨白细胞介素（ＩＬ）－２与ＩＬ－４对膀胱癌肿瘤浸润性淋巴细胞（ＴＩＬ）体外增殖及细胞毒性免疫调控的协同作用。方法 分离膀胱癌ＴＩＬ，置于含ＩＬ－２和（或）ＩＬ－２的完全培养基因中培养４周，定期计数ＴＩＬ增殖数量。四甲基偶氮唑蓝（ＭＴＴ）比色法检测ＴＩＬ细胞毒性。结果 对比单纯ＩＬ－２的培养条件，ＩＬ－２联合ＩＬ－４后４周时ＴＩＬ扩增数量是前者的１．６５倍（Ｐ〈０．０５）。在交靶比为１０：１时，ＴＩＬ对自体膀胱癌细胞（ＢＴＴ７３９）表现出高水平的杀伤活性（Ｐ〈０．０５）。联合培养的ＴＩＬ抗ＢＴＴ３９或小鼠淋巴瘤瘤株（ＹＡＣ－１）的活性与在单纯ＩＬ－２培养的条件下相比无显著改变（Ｐ均〉０．０５）。结论 ＩＬ－４对ＩＬ－２活化的膀胱癌ＴＩＬ增殖具有较强的正向调节效应，而对ＴＩＬ细胞毒性未见明显影响。

The synergy effects of IL-2 and IL-4 on the proliferation and cytotoxicity of tumor-infiltrating lymphocytes from murine bladder carcinoma

Objective　To investigate the synergy effects of IL-2 and IL-4 on the proliferation and cytotoxicity of tumor-infiltrating lymphocytes (TIL) from murine bladder carcinoma in vitro.　Methods　 Suspensions of TIL were prepared from T739 murine bladder carcinoma.All cultures were incubated in RPMI-1640 complete media with IL-2 and /or IL-4. During 4 weeks of culture,TILs were counted regularly.The cytotoxicity of TILs was measured using MTT colorimetric assay.　Results　The fold expansions of TILs in IL-2 plus IL-4 for 4 weeks was 1.65 times higher than that in IL-2 alone (P<0.05).The cytotoxic activity of TIL against autologous BTT739 cells was stronger than that against YAC-1 tumor cell line at an E/T ration of 10∶1(P<0.05).IL-2 in conjunction with IL-4 could not preferentially augment the cytolysis of TIL to BTT739 cancer cells or YAC-1 tumor cell line than TIL growed in IL-2 alone did (P>0.05 ).　Conclusion　IL-4 can significantly promote the proliferation of bladder carcinoma TILs activated by IL-2, while exhibite no statistically significant effect on the cytotoxicity of TIL.