关于玉米中玉米赤霉烯酮(ZEA)阳性结果确证的研究

目的 15批玉米样品检测玉米赤霉烯酮,其中有2批样品疑似阳性。通过本实验对结果进行确证。方法比较流动相采用等度洗脱(乙腈:水=65:35)时重复进标准样品和疑似样品对保留时间的稳定性影响,采用等度洗脱时不同流动相配比(乙腈:水分别为65:35和55:45)时标准样品的出峰时间,以及流动相采用等度洗脱(乙腈:水=55:45)时,不同溶解液配比(乙腈:水为65:35和乙腈:水:甲醇为46:46:8)标准品的出峰时间。结果排除干扰,确定此两批玉米样品中玉米赤霉烯酮呈阳性。结论多方面对疑似阳性结果进行确证,为日常玉米样品的检测提供了可靠、科学的验证依据。

Study on confirmation of positive results of zearalenone (ZEA) in corn

Objective To confirm 2 out of 15 corn samples which were suspected zearalenone positive. Methods The stability of runtime between standard samples and suspected samples was compared when the mobile phase adopted isocratic elution (acetonitrile:water=65:35). The peak time of standard samples was compared when the mobile phase adopted different mobile phase ratio (acetonitrile:water =65:35 vs 55:45). And the peak time of standard samples was compared using different solvents when the mobile phase adopted isocratic elution (acetonitrile:water=55:45). Results The two batches of corn samples were positive for zearalenone. Conclusion This study provides a reliable scientific method for the detection of corn samples.